{"doi":"10.1165/rcmb.2024-0633ma","title":"Ozone Generation Method Impacts Lung Toxicity and Oxidant Signaling","abstract":"Ozone (O3) is a criteria pollutant that is anticipated to increase over the next decade because of climate-related activity. Varying amounts of nitrogen oxides (NOx) are produced as byproducts during O3 generation from oxygen depending on the method of production, including the source and oxygen purity. A review of the current literature confirms a lack of consistent monitoring and reporting of potential nitrogen species produced with different methods of experimental O3 generation. The lack of consistent monitoring and reporting is potentially a factor that can explain divergence of reported experimental O3 exposure outcomes from different research groups. In the present report, we compare the effects of O3 generation from either a filtered air (FA-ozone) or a pure oxygen (Oxy-ozone) source on NOx generation and measures of O3-induced lung injury. We also consider if this impacts mixed exposures with O3 and ultrafine carbon black (CB) based on if the O3 was generated from a filtered air (FA-ozone-CB) versus a pure oxygen (Oxy-ozone-CB) source. Comparing FA-ozone versus Oxy-ozone, we observed increased lung inflammation and injury in the FA-ozone group. In the FA-ozone-CB group, compared with the Oxy-ozone-CB group, the FA-ozone-CB inhalation exposure resulted in the formation of a greater amount of NOx and induced protein nitrotyrosine in the lungs. Moreover, the FA-ozone-CB group had evidence of eosinophil recruitment not observed in the Oxy-ozone-CB group. Overall, this suggests that the source of oxygen for O3 generation impacts experimental outcomes. Furthermore, measurement and reporting of nitrogen species in O3 exposure should be considered.","journal":"American Journal of Respiratory Cell and Molecular Biology","year":2025,"id":525951,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9494,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":749852,"name":"Md Habibul Hasan Mazumder","orcid":"0000-0002-7807-8372","position":1,"is_corresponding":false},{"id":453101,"name":"William T. Goldsmith","orcid":"0000-0002-6983-0200","position":2,"is_corresponding":false},{"id":1401474,"name":"Ning-Hua Tan","orcid":null,"position":3,"is_corresponding":false},{"id":348504,"name":"Anand Ranpara","orcid":"0000-0003-3723-2537","position":4,"is_corresponding":false},{"id":368819,"name":"Vamsi Kodali","orcid":"0000-0001-6177-0568","position":5,"is_corresponding":false},{"id":313064,"name":"Murugesan Velayutham","orcid":null,"position":6,"is_corresponding":false},{"id":1401039,"name":"Qiang Wang","orcid":"0000-0002-1615-662X","position":7,"is_corresponding":false},{"id":292273,"name":"Robert M. Tighe","orcid":"0000-0002-3465-9861","position":8,"is_corresponding":false},{"id":292268,"name":"Salik Hussain","orcid":"0000-0003-0339-6226","position":9,"is_corresponding":false},{"id":239202,"name":"Atefeh Razazan","orcid":null,"position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":null,"created_at":"2026-07-19T02:50:25.860105Z","pmid":"40488720","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}