{"doi":"10.1161/circresaha.125.326156","title":"High-Throughput Echocardiography-Guided Induction of Myocardial Ischemia/Reperfusion in Mice","abstract":"BACKGROUND: Mouse models of myocardial ischemia with subsequent heart failure are common approaches to examine heart failure pathology and possible treatment strategies. We sought to establish a high-throughput approach for echocardiography-guided induction of myocardial ischemia/reperfusion (IR) in mice. METHODS: After visualization of the left coronary artery with high-resolution ultrasound imaging and echocardiographic definition of the level of coronary occlusion, the left anterior descending artery was temporarily occluded with 2 micromanipulator-controlled needles. Functional and molecular changes were assessed and compared with commonly performed surgical techniques. RESULTS: Echocardiography-guided induction of myocardial IR enabled standardized induction of myocardial IR injury with subsequent left ventricular remodeling. Incorporation of various quality control measures throughout the procedure ensured a high success rate and the absence of relevant postinterventional mortality in experienced hands. Compared with surgical approaches, echocardiography-guided induction of myocardial IR showed a quicker recovery time and induced a less pronounced inflammatory response characterized by decreased local and systemic neutrophil counts. Notably, infarct size and subsequent post-myocardial infarction cardiac dysfunction were comparable between methods. The novel procedure was successfully implemented at different academic institutions with imaging expertise and demonstrated high interinstitutional reproducibility. CONCLUSIONS: Echocardiography-guided induction of myocardial IR enables high-throughput induction of myocardial IR injury with precise echocardiographic definition of the occlusion level and immediate evaluation of cardiac function during ischemia. The method provides a more clinically relevant assessment of IR sequelae and offers notable animal welfare advantages by eliminating the need for ventilation and thoracotomy, thereby mitigating potential surgery-related confounders.","journal":"Circulation Research","year":2025,"id":515616,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9547,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1227189,"name":"Niklas Hartmann","orcid":"0009-0009-1799-7162","position":1,"is_corresponding":false},{"id":753236,"name":"Tibor Kovács","orcid":"0000-0002-0632-9128","position":2,"is_corresponding":false},{"id":258404,"name":"Carla J. Weinheimer","orcid":"0000-0001-7922-7512","position":3,"is_corresponding":false},{"id":1380539,"name":"Jess Nigro","orcid":null,"position":4,"is_corresponding":false},{"id":1380540,"name":"Tobias Thiemann","orcid":null,"position":5,"is_corresponding":false},{"id":867739,"name":"Junedh Amrute","orcid":"0000-0002-6851-0168","position":6,"is_corresponding":false},{"id":1379952,"name":"David Schumacher","orcid":"0000-0002-2308-6543","position":7,"is_corresponding":false},{"id":1380541,"name":"Moritz Kornardt","orcid":null,"position":8,"is_corresponding":false},{"id":1379953,"name":"Laura M. Wienecke","orcid":"0000-0001-7587-8422","position":9,"is_corresponding":false},{"id":1380542,"name":"Lennart Rompel","orcid":null,"position":10,"is_corresponding":false},{"id":1318233,"name":"Johannes Fischer","orcid":"0009-0000-4327-1079","position":11,"is_corresponding":false},{"id":314332,"name":"John F. Bachman","orcid":"0000-0002-2912-9911","position":12,"is_corresponding":false},{"id":813200,"name":"Olivia Bedard","orcid":"0009-0008-7888-1895","position":13,"is_corresponding":false},{"id":246480,"name":"Shibali Das","orcid":"0000-0003-1263-8178","position":14,"is_corresponding":false},{"id":1379954,"name":"Tim Christian Kuhn","orcid":"0000-0003-2357-1335","position":15,"is_corresponding":false},{"id":394516,"name":"Mirko Völkers","orcid":"0000-0003-2344-1856","position":16,"is_corresponding":false},{"id":278720,"name":"Ralf P. Brandes","orcid":"0000-0002-8035-0048","position":17,"is_corresponding":false},{"id":350123,"name":"Rafael Kramann","orcid":"0000-0003-4048-6351","position":18,"is_corresponding":false},{"id":268613,"name":"Nadia Rosenthal","orcid":"0000-0002-7599-7365","position":19,"is_corresponding":false},{"id":28416,"name":"Norbert Frey","orcid":"0000-0001-7611-378X","position":20,"is_corresponding":false},{"id":247530,"name":"Kory J. Lavine","orcid":"0000-0003-1948-9945","position":21,"is_corresponding":false},{"id":552530,"name":"Florian Leuschner","orcid":"0000-0003-1157-474X","position":22,"is_corresponding":false},{"id":1379951,"name":"Florian Sicklinger","orcid":"0009-0004-6707-1695","position":0,"is_corresponding":true}],"reference_count":18,"raw_metadata":null,"created_at":"2026-07-19T02:48:39.436300Z","pmid":"40207377","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}