{"doi":"10.1161/circimaging.119.009889","title":"CCR2 Positron Emission Tomography for the Assessment of Abdominal Aortic Aneurysm Inflammation and Rupture Prediction","abstract":"Background: The monocyte chemoattractant protein-1/CCR2 (chemokine receptor 2) axis plays an important role in abdominal aortic aneurysm (AAA) pathogenesis, with effects on disease progression and anatomic stability. We assessed the expression of CCR2 in a rodent model and human tissues, using a targeted positron emission tomography radiotracer ( 64 Cu-DOTA-ECL1i). Methods: AAAs were generated in Sprague-Dawley rats by exposing the infrarenal, intraluminal aorta to PPE (porcine pancreatic elastase) under pressure to induce aneurysmal degeneration. Heat-inactivated PPE was used to generate a sham operative control. Rat AAA rupture was stimulated by the administration of β-aminopropionitrile, a lysyl oxidase inhibitor. Biodistribution was performed in wild-type rats at 1 hour post tail vein injection of 64 Cu-DOTA-ECL1i. Dynamic positron emission tomography/computed tomography imaging was performed in rats to determine the in vivo distribution of radiotracer. Results: Biodistribution showed fast renal clearance. The localization of radiotracer uptake in AAA was verified with high-resolution computed tomography. At day 7 post-AAA induction, the radiotracer uptake (standardized uptake value [SUV]=0.91±0.25) was approximately twice that of sham-controls (SUV=0.47±0.10; P <0.01). At 14 days post-AAA induction, radiotracer uptake by either group did not significantly change (AAA SUV=0.86±0.17 and sham-control SUV=0.46±0.10), independent of variations in aortic diameter. Competitive CCR2 receptor blocking significantly decreased AAA uptake (SUV=0.42±0.09). Tracer uptake in AAAs that subsequently ruptured (SUV=1.31±0.14; P <0.005) demonstrated uptake nearly twice that of nonruptured AAAs (SUV=0.73±0.11). Histopathologic characterization of rat and human AAA tissues obtained from surgery revealed increased expression of CCR2 that was co-localized with CD68 + macrophages. Ex vivo autoradiography demonstrated specific binding of 64 Cu-DOTA-ECL1i to CCR2 in both rat and human aortic tissues. Conclusions: CCR2 positron emission tomography is a promising new biomarker for the noninvasive assessment of AAA inflammation that may aid in associated rupture prediction.","journal":"Circulation Cardiovascular Imaging","year":2020,"id":66797,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":49,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9608,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":354865,"name":"Sergio E. Sastriques","orcid":null,"position":1,"is_corresponding":false},{"id":353784,"name":"Lisa Detering","orcid":"0000-0001-7061-1024","position":2,"is_corresponding":false},{"id":354866,"name":"Deborah Sultan","orcid":null,"position":3,"is_corresponding":false},{"id":273845,"name":"Hannah Luehmann","orcid":"0000-0002-7516-6772","position":4,"is_corresponding":false},{"id":353785,"name":"Batool Arif","orcid":"0009-0006-3610-0865","position":5,"is_corresponding":false},{"id":273847,"name":"Gyu Seong Heo","orcid":"0000-0001-6200-4742","position":6,"is_corresponding":false},{"id":353786,"name":"Xiaohui Zhang","orcid":"0000-0002-9259-5093","position":7,"is_corresponding":false},{"id":273852,"name":"Richard Laforest","orcid":"0000-0002-0238-1439","position":8,"is_corresponding":false},{"id":353787,"name":"Jie Zheng","orcid":"0000-0003-4958-2091","position":9,"is_corresponding":false},{"id":282754,"name":"Chieh‐Yu Lin","orcid":"0000-0002-4269-3234","position":10,"is_corresponding":false},{"id":273858,"name":"Robert J. Gropler","orcid":"0000-0002-6550-0570","position":11,"is_corresponding":false},{"id":273860,"name":"Yongjian Liu","orcid":"0000-0002-1118-1535","position":12,"is_corresponding":false},{"id":354864,"name":"Sean J. English","orcid":null,"position":0,"is_corresponding":true}],"reference_count":43,"raw_metadata":null,"created_at":"2026-07-18T21:14:50.590588Z","pmid":"32164451","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}