{"doi":"10.1161/atvbaha.114.303533","title":"Impact of Phospholipid Transfer Protein on Nascent High-Density Lipoprotein Formation and Remodeling","abstract":"<jats:sec>\n            <jats:title>Objective—</jats:title>\n            <jats:p>Phospholipid transfer protein (PLTP), which binds phospholipids and facilitates their transfer between lipoproteins in plasma, plays a key role in lipoprotein remodeling, but its influence on nascent high-density lipoprotein (HDL) formation is not known. The effect of PLTP overexpression on apolipoprotein A-I (apoA-I) lipidation by primary mouse hepatocytes was investigated.</jats:p>\n          </jats:sec>\n          <jats:sec>\n            <jats:title>Approach and Results—</jats:title>\n            <jats:p>Overexpression of PLTP through an adenoviral vector markedly affected the amount and size of lipidated apoA-I species that were produced in hepatocytes in a dose-dependent manner, ultimately generating particles that were &lt;7.1 nm but larger than lipid-free apoA-I. These &lt;7.1-nm small particles generated in the presence of overexpressed PLTP were incorporated into mature HDL particles more rapidly than apoA-I both in vivo and in vitro and were less rapidly cleared from mouse plasma than lipid-free apoA-I. The &lt;7.1-nm particles promoted both cellular cholesterol and phospholipid efflux in an ATP-binding cassette transporter A1–dependent manner, similar to apoA-I in the presence of PLTP. Lipid-free apoA-I had a greater efflux capacity in the presence of PLTP than in the absence of PLTP, suggesting that PLTP may promote ATP-binding cassette transporter A1–mediated cholesterol and phospholipid efflux. These results indicate that PLTP alters nascent HDL formation by modulating the lipidated species and by promoting the initial process of apoA-I lipidation.</jats:p>\n          </jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions—</jats:title>\n            <jats:p>Our findings suggest that PLTP exerts significant effects on apoA-I lipidation and nascent HDL biogenesis in hepatocytes by promoting ATP-binding cassette transporter A1–mediated lipid efflux and the remodeling of nascent HDL particles.</jats:p>\n          </jats:sec>","journal":"Arteriosclerosis, Thrombosis, and Vascular Biology","year":2014,"id":31522,"datarank":1.1975337007224094,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"self_citation_contribution":0.47670807455219194,"citation_network_contribution":0.7208256261702174,"self_endowment_contribution":0.47670807455219194,"citer_contribution":0.7208256261702174,"corpus_percentile":null,"corpus_rank":null,"citation_count":23,"citer_count":22,"citers_with_citation_signal":18,"citers_with_endowment":18,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":168762,"name":"Joanne M. Wroblewski","orcid":null,"position":1,"is_corresponding":false},{"id":168763,"name":"Nancy R. Webb","orcid":null,"position":2,"is_corresponding":false},{"id":167824,"name":"Deneys R. van der Westhuyzen","orcid":null,"position":3,"is_corresponding":false},{"id":168761,"name":"Ailing Ji","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25060793","pmcid":"PMC4141034","openalex_id":"https://openalex.org/W2163091262","authors":[],"funders":[{"funder_name":"NHLBI NIH HHS","grant_id":"P01HL086670","title":null},{"funder_name":"CSRD VA","grant_id":"I01 CX000773","title":null}],"total_grants":2,"fwci":2.0991,"citation_percentile":0.87455016,"influential_citations":1,"citation_trend":[{"year":2015,"count":4},{"year":2016,"count":2},{"year":2017,"count":4},{"year":2018,"count":3},{"year":2019,"count":3},{"year":2020,"count":1},{"year":2022,"count":1},{"year":2023,"count":3},{"year":2026,"count":2}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://www.ahajournals.org/doi/pdf/10.1161/ATVBAHA.114.303533","host_type":"journal"},{"url":"https://www.ahajournals.org/doi/pdf/10.1161/ATVBAHA.114.303533","host_type":"BRONZE"},{"url":"https://www.ahajournals.org/doi/pdf/10.1161/ATVBAHA.114.303533","host_type":"publisher"},{"url":"https://www.ahajournals.org/doi/full/10.1161/ATVBAHA.114.303533","host_type":"publisher"},{"url":"https://doi.org/10.1161/atvbaha.114.303533","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25060793","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4141034","host_type":"repository"}],"fields_of_study":["Diabetes, Cardiovascular Risks, and Lipoproteins","Cholesterol and Lipid Metabolism","Lipoproteins and Cardiovascular Health","Medicine","Biology","Chemistry","ATP Binding Cassette Transporter 1","Adenoviridae","Animals","Apolipoprotein A-I","Cells, Cultured","Cholesterol","Gene Expression Regulation","Genetic Vectors","Hepatocytes","Kidney","Mice","Mice, Inbred C57BL","Mice, Transgenic","Phospholipid Transfer Proteins","Phospholipids","Recombinant Fusion Proteins"],"mesh_terms":["Adenoviridae","Animals","Cells, Cultured","Cholesterol","Gene Expression Regulation","Genetic Vectors","Kidney","Mice, Inbred C57BL","Mice, Transgenic","Phospholipids","Recombinant Fusion Proteins","Apolipoprotein A-I","Hepatocytes","Phospholipid Transfer Proteins","Mice","ATP Binding Cassette Transporter 1"],"keywords":["Phospholipid transfer protein","Lipid-anchored protein","Apolipoprotein B","Phospholipid","ABCA1","Chemistry","Lipoprotein","Efflux","Cholesterol","Reverse cholesterol transport","Biochemistry","Cell biology","Transporter","High-density lipoprotein","Biology","Membrane","Autophagy","Gene","Apoptosis","Hepatocytes","Nascent Hdl"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T07:26:14.205281Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}