{"doi":"10.1159/000539177","title":"Murine Ribonuclease 6 Limits Bacterial Dissemination During Experimental Urinary Tract Infection","abstract":"INTRODUCTION: The ribonuclease (RNase) A superfamily encodes cationic antimicrobial proteins with potent microbicidal activity toward uropathogenic bacteria. Ribonuclease 6 (RNase6) is an evolutionarily conserved, leukocyte-derived antimicrobial peptide with potent microbicidal activity toward uropathogenic Escherichia coli (UPEC), the most common cause of bacterial urinary tract infections (UTIs). In this study, we generated Rnase6-deficient mice to investigate the hypothesis that endogenous RNase 6 limits host susceptibility to UTI. METHODS: We generated a Rnase6EGFP knock-in allele to identify cellular sources of Rnase6 and determine the consequences of homozygous Rnase6 deletion on antimicrobial activity and UTI susceptibility. RESULTS: We identified monocytes and macrophages as the primary cellular sources of Rnase6 in bladders and kidneys of Rnase6EGFP/+ mice. Rnase6 deficiency (i.e., Rnase6EGFP/EGFP) resulted in increased upper urinary tract UPEC burden during experimental UTI, compared to Rnase6+/+ controls. UPEC displayed increased intracellular survival in Rnase6-deficient macrophages. CONCLUSION: Our findings establish that RNase6 prevents pyelonephritis by promoting intracellular UPEC killing in monocytes and macrophages and reinforce the overarching contributions of endogenous antimicrobial RNase A proteins to host UTI defense.","journal":"Journal of Innate Immunity","year":2024,"id":465507,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9635,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1114191,"name":"Macie M. Kercsmar","orcid":null,"position":1,"is_corresponding":false},{"id":1063964,"name":"Birong Li","orcid":"0000-0001-9214-3890","position":2,"is_corresponding":false},{"id":1022741,"name":"Gabriela Vásquez Martínez","orcid":"0000-0003-3239-3737","position":3,"is_corresponding":false},{"id":428874,"name":"Sudipti Gupta","orcid":"0000-0001-7574-5064","position":4,"is_corresponding":false},{"id":428882,"name":"Christina B. Ching","orcid":"0000-0002-3396-4215","position":5,"is_corresponding":false},{"id":700251,"name":"Gregory A. Ballash","orcid":"0000-0002-0050-6154","position":6,"is_corresponding":false},{"id":1063962,"name":"Israel Cotzomi-Ortega","orcid":"0000-0001-5627-2839","position":7,"is_corresponding":false},{"id":1063963,"name":"Yuriko I. Sánchez-Zamora","orcid":"0000-0002-0769-8523","position":8,"is_corresponding":false},{"id":1113674,"name":"Ester Boix","orcid":"0000-0003-1790-2142","position":9,"is_corresponding":false},{"id":371821,"name":"Diana Zepeda‐Orozco","orcid":"0000-0003-0671-4453","position":10,"is_corresponding":false},{"id":428876,"name":"Ashley R. Jackson","orcid":"0000-0002-1571-3906","position":11,"is_corresponding":false},{"id":699566,"name":"John David Spencer","orcid":"0000-0001-5509-7135","position":12,"is_corresponding":false},{"id":421678,"name":"Juan de Dios Ruiz‐Rosado","orcid":"0000-0002-6429-9086","position":13,"is_corresponding":false},{"id":428881,"name":"Brian Becknell","orcid":"0000-0002-2750-4415","position":14,"is_corresponding":false},{"id":1114190,"name":"Hanna H. Cortado","orcid":null,"position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":null,"created_at":"2026-07-19T02:04:50.328230Z","pmid":"38744252","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}