{"doi":"10.1159/000519618","title":"Mapping Sequential IgE-Binding Epitopes on Major and Minor Egg Allergens","abstract":"<b><i>Introduction:</i></b> Molecular studies of hen’s egg allergens help define allergic phenotypes, with IgE to sequential (linear) epitopes on the ovomucoid (OVM) protein associated with a persistent disease. Epitope profiles of other egg allergens are largely unknown. The objective of this study was to construct an epitope library spanning across 7 allergens and further evaluate sequential epitope-specific (<i>ses-</i>)IgE and <i>ses-</i>IgG<sub>4</sub> among baked-egg reactive or tolerant children. <b><i>Methods:</i></b> A Bead-Based Epitope Assay was used to identify informative IgE epitopes from 15-mer overlapping peptides covering the entire OVM and ovalbumin (OVA) proteins in 38 egg allergic children. An amalgamation of 12 B-cell epitope prediction tools was developed using experimentally identified epitopes. This ensemble was used to predict epitopes from ovotransferrin, lysozyme, serum albumin, vitellogenin-II fragment, and vitellogenin-1 precursor. <i>Ses-</i>IgE and <i>ses-</i>IgG<sub>4</sub> repertoires of 135 egg allergic children (82 reactive to baked-egg, the remaining 52 tolerant), 46 atopic controls, and 11 healthy subjects were compared. <b><i>Results:</i></b> 183 peptides from OVM and OVA were screened and used to create an aggregate algorithm, improving predictions of 12 individual tools. A final library of 65 sequential epitopes from 7 proteins was constructed. Egg allergic children had higher <i>ses-</i>IgE and lower <i>ses-</i>IgG<sub>4</sub> to predominantly OVM epitopes than both atopic and healthy controls. Baked-egg reactive children had similar <i>ses-</i>IgG<sub>4</sub> but greater <i>ses-</i>IgE than tolerant group. A combination of OVA-sIgE with <i>ses-</i>IgEs to OVM-023 and OVA-028 was the best predictor of reactive phenotype. <b><i>Conclusion:</i></b> We have created a comprehensive epitope library and showed that <i>ses-</i>IgE is a potential biomarker of baked-egg reactivity.","journal":"International Archives of Allergy and Immunology","year":2021,"id":166458,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9446,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":241620,"name":"Scott H. Sicherer","orcid":"0000-0003-0036-0439","position":1,"is_corresponding":false},{"id":110402,"name":"Robert A. Wood","orcid":"0000-0003-3777-802X","position":2,"is_corresponding":false},{"id":280637,"name":"Stacie M. Jones","orcid":"0000-0003-3491-4815","position":3,"is_corresponding":false},{"id":227789,"name":"Donald Y.M. Leung","orcid":"0000-0002-0177-3844","position":4,"is_corresponding":false},{"id":273995,"name":"A. Wesley Burks","orcid":"0000-0003-1873-5702","position":5,"is_corresponding":false},{"id":265882,"name":"David Dunkin","orcid":"0000-0002-7921-134X","position":6,"is_corresponding":false},{"id":692996,"name":"Marc Witmer","orcid":null,"position":7,"is_corresponding":false},{"id":312710,"name":"Galina Grishina","orcid":null,"position":8,"is_corresponding":false},{"id":586373,"name":"Robert Getts","orcid":null,"position":9,"is_corresponding":false},{"id":18943,"name":"Mayte Suárez‐Fariñas","orcid":"0000-0001-8712-3553","position":10,"is_corresponding":false},{"id":273996,"name":"Hugh A. Sampson","orcid":"0000-0003-1613-8875","position":11,"is_corresponding":false},{"id":105279,"name":"Maria Suprun","orcid":"0000-0001-9161-4021","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":null,"created_at":"2026-07-18T23:45:49.644768Z","pmid":"34818647","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}