{"doi":"10.1159/000350869","title":"Physical Mapping of 20 Unmapped Fragments of the Btau_4.0 Genome Assembly in Cattle, Sheep and River Buffalo","abstract":"<jats:p>The recent advances in sequencing technology and bioinformatics have revolutionized genomic research, making the decoding of the genome an easier task. Genome sequences are currently available for many species, including cattle, sheep and river buffalo. The available reference genomes are very accurate, and they represent the best possible order of loci at this time. In cattle, despite the great accuracy achieved, a part of the genome has been sequenced but not yet assembled: these genome fragments are called unmapped fragments. In the present study, 20 unmapped fragments belonging to the Btau_4.0 reference genome have been mapped by FISH in cattle (&lt;i&gt;Bos taurus&lt;/i&gt;, 2n = 60), sheep (&lt;i&gt;Ovis aries&lt;/i&gt;, 2n = 54) and river buffalo (&lt;i&gt;Bubalus bubalis&lt;/i&gt;, 2n = 50). Our results confirm the accuracy of the available reference genome, though there are some discrepancies between the expected localization and the observed localization. Moreover, the available data in the literature regarding genomic homologies between cattle, sheep and river buffalo are confirmed. Finally, the results presented here suggest that FISH was, and still is, a useful technology to validate the data produced by genome sequencing programs.</jats:p>","journal":"Cytogenetic and Genome Research","year":2013,"id":13630,"datarank":0.33928677775082294,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.04740025539252593,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.04740025539252593,"corpus_percentile":48.541811711920786,"corpus_rank":6653,"citation_count":6,"citer_count":1,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":111050,"name":"V. Genualdo","orcid":null,"position":1,"is_corresponding":false},{"id":111051,"name":"A. Perucatti","orcid":null,"position":2,"is_corresponding":false},{"id":111052,"name":"A. Iannuzzi","orcid":null,"position":3,"is_corresponding":false},{"id":111053,"name":"L. Iannuzzi","orcid":null,"position":4,"is_corresponding":false},{"id":111054,"name":"P. Parma","orcid":null,"position":5,"is_corresponding":false},{"id":111049,"name":"L. De Lorenzi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23652984","pmcid":null,"openalex_id":"https://openalex.org/W2066142713","authors":[],"funders":[],"total_grants":0,"fwci":0.1496,"citation_percentile":0.57146514,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2020,"count":2},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":1}],"oa_status":"closed","license":"https://www.karger.com/Services/SiteLicenses","oa_locations":[{"url":"https://www.karger.com/Article/Pdf/350869","host_type":"publisher"},{"url":"https://doi.org/10.1159/000350869","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23652984","host_type":"repository"}],"fields_of_study":["Genomics and Phylogenetic Studies","Chromosomal and Genetic Variations","Genetic Mapping and Diversity in Plants and Animals","Biology","Medicine","Agricultural and Food Sciences","Animals","Base Sequence","Buffaloes","Cattle","Chromosomes, Artificial, Bacterial","Chromosomes, Mammalian","Databases, Genetic","Genetic Loci","Genome","In Situ Hybridization, Fluorescence","Interleukin-13 Receptor alpha2 Subunit","Physical Chromosome Mapping","Reproducibility of Results","Sequence Homology, Nucleic Acid","Sheep","Species Specificity"],"mesh_terms":["Animals","Base Sequence","Buffaloes","Cattle","Sequence Homology, Nucleic Acid","Sheep","Species Specificity","Reproducibility of Results","Genome","In Situ Hybridization, Fluorescence","Physical Chromosome Mapping","Chromosomes, Artificial, Bacterial","Databases, Genetic","Chromosomes, Mammalian","Interleukin-13 Receptor alpha2 Subunit","Genetic Loci"],"keywords":["Genome","Biology","Reference genome","Bovine genome","Bubalus","Genetics","Sequence assembly","Water buffalo","Ovis","Computational biology","Gene","Veterinary medicine"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.5124991.v1","title":"Supplementary Material for: Physical Mapping of 20 Unmapped Fragments of the Btau_4.0 Genome Assembly in Cattle, Sheep and River Buffalo","publisher":"Karger Publishers","resource_type":"Dataset"},{"doi":"10.6084/m9.figshare.5124991","title":"Supplementary Material for: Physical Mapping of 20 Unmapped Fragments of the Btau_4.0 Genome Assembly in Cattle, Sheep and River Buffalo","publisher":"Karger Publishers","resource_type":"Dataset"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-05-31T06:35:44.199624Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}