{"doi":"10.1159/000139275","title":"Inhibition of Leukotriene Production by FK506 in Rat Basophilic Leukemia-1 Cells","abstract":"<jats:p>We investigated the inhibitory action of FK506 (0.0005-5 µg/ml) on the metabolism of arachidonate 5-lipoxygenase in rat basophilic leukemia-1 cells. Cells were stimulated with A23187 (10-5 mol/l) for 15 min in the absence or presence of various concentrations of FK506. Arachidonate 5-lipoxygenase metabolites, peptide leukotrienes (LTs), leukotriene B&lt;sub&gt;4 &lt;/sub&gt;(LTB&lt;sub&gt;4&lt;/sub&gt;) and 5-hydroxyeicosatetraenoic acid (5-HETE) were measured by high-performance liquid chromatography. FK506 inhibited A23187-stim-ulated production of peptide LTs, LTB4 and 5-HETE in intact cells by up to 77, 73 and 60%, respectively. Phospholipase A&lt;sub&gt;2&lt;/sub&gt; activity, measured by the release of 3H-achidonic acid (AA), was not significantly inhibited by FK506. The synthesis of peptide LTs and LTB4 was not inhibited by FK506 when leukotriene A&lt;sub&gt;4&lt;/sub&gt;-free acid was added to the culture medium. The synthesis of peptide LTs, LTB4 and 5-HETE was not affected by FK506 in a cell lysate study using AA as the subtrate. These results indicate that FK506 inhibits the production of peptide LTs, LTB&lt;sub&gt;4&lt;/sub&gt; and 5-HETE by inhibiting 5-lipoxygenase activity in intact cells. The inhibition is not a direct action on 5-lipoxygenase but results from the activating processes of this enzyme.</jats:p>","journal":"Pharmacology","year":2008,"id":32296,"datarank":0.8938630531207823,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.5484752891716753,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.5484752891716753,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":9,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":171258,"name":"Ikuko Kobayashi","orcid":null,"position":1,"is_corresponding":false},{"id":171259,"name":"Shigetaka Matsumoto","orcid":null,"position":2,"is_corresponding":false},{"id":171260,"name":"Masafumi Zaitu","orcid":null,"position":3,"is_corresponding":false},{"id":171261,"name":"Eriko Muro","orcid":null,"position":4,"is_corresponding":false},{"id":171262,"name":"Tomohiro Ichimaru","orcid":null,"position":5,"is_corresponding":false},{"id":171263,"name":"Sumio Miyazaki","orcid":null,"position":6,"is_corresponding":false},{"id":171257,"name":"Yuhei Hamasaki","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.302585092994046,"endowment":2.302585092994046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7538218","pmcid":null,"openalex_id":"https://openalex.org/W2082721898","authors":[],"funders":[],"total_grants":0,"fwci":0.1515,"citation_percentile":0.56684132,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2018,"count":1}],"oa_status":"closed","license":"https://karger.com/pages/terms-and-conditions","oa_locations":[{"url":"https://karger.com/article-pdf/doi/10.1159/000139275","host_type":"publisher"},{"url":"https://doi.org/10.1159/000139275","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7538218","host_type":"repository"}],"fields_of_study":["Chemokine receptors and signaling","Cytokine Signaling Pathways and Interactions","Immune Response and Inflammation","Chemistry","Medicine","Animals","Hydroxyeicosatetraenoic Acids","Leukemia, Basophilic, Acute","Leukotrienes","Lipoxygenase Inhibitors","Phospholipases A","Phospholipases A2","Rats","Tacrolimus","Tumor Cells, Cultured"],"mesh_terms":["Animals","Hydroxyeicosatetraenoic Acids","Phospholipases A","Tumor Cells, Cultured","Leukotrienes","Leukemia, Basophilic, Acute","Tacrolimus","Lipoxygenase Inhibitors","Rats","Phospholipases A2"],"keywords":["Arachidonate 5-lipoxygenase","Leukotriene B4","Arachidonic acid","Lipoxygenase","Peptide","Chemistry","Leukotriene","Phospholipase A2","Hydroxyeicosatetraenoic acid","Leukotriene C4","Formyl peptide receptor","Biochemistry","Cell culture","Enzyme","Biology","Receptor","Inflammation","Immunology","Chemotaxis"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T11:18:20.609983Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}