{"doi":"10.1159/000022536","title":"Characterization of Murine Anti-Glycoprotein Ib Monoclonal Antibodies That Differentiate between Shear-Induced and Ristocetin/Botrocetin-Induced Glycoprotein Ib-von Willebrand Factor Interaction","abstract":"<jats:p>Platelet adhesion to vascular subendothelium under conditions of high shear stress is mediated by the platelet glycoprotein (GP) Ib-von Willebrand Factor (vWF) interaction. The aim of this study was to characterize the murine monoclonal antibodies (MoAbs) 27A10 and 28E6, both raised against purified GPIb. The MoAb 27A10 is a potent inhibitor of shear-induced platelet adhesion to collagen type I in a flow chamber at shear rates of 1,300 and 2,700 s&lt;sup&gt;–1&lt;/sup&gt;. 20 μg/ml of MoAb 27A10, furthermore, could completely block shear-induced aggregation in a modified Couette viscometer at shear rates of 1,000 and 4,000 s&lt;sup&gt;–1&lt;/sup&gt;. On the other hand, MoAb 27A10 had a negligible effect on botrocetin-induced GPIb-vWF binding and is only a poor inhibitor of the ristocetin-dependent interaction. In contrast, MoAb 28E6 did abolish both the ristocetin- and botrocetin-induced GPIb-vWF binding, whereas it did not block the shear-induced interaction. Thus, we identify here two anti-GPIb MoAbs 27A10 and 28E6 that either preferentially inhibit the shear-induced or the ristocetin/botrocetin-induced platelet-vWF interaction. With these tools it should be possible to more clearly define the mechanisms by which platelets bind to vWF in vivo.</jats:p>","journal":"Pathophysiology of Haemostasis and Thrombosis","year":2000,"id":34635,"datarank":0.5505579522748085,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.17782195480660845,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.17782195480660845,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":3,"citers_with_citation_signal":3,"citers_with_endowment":3,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":179082,"name":"N. Ajzenberg","orcid":null,"position":1,"is_corresponding":false},{"id":179083,"name":"S. Vauterin","orcid":null,"position":2,"is_corresponding":false},{"id":179084,"name":"M.F. Hoylaerts","orcid":null,"position":3,"is_corresponding":false},{"id":179085,"name":"P.J. Declerck","orcid":null,"position":4,"is_corresponding":false},{"id":179086,"name":"D. Baruch","orcid":null,"position":5,"is_corresponding":false},{"id":179087,"name":"H. Deckmyn","orcid":null,"position":6,"is_corresponding":false},{"id":179081,"name":"N. Cauwenberghs","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11014964","pmcid":null,"openalex_id":"https://openalex.org/W2153168033","authors":[],"funders":[],"total_grants":0,"fwci":0.7635,"citation_percentile":0.70829572,"influential_citations":0,"citation_trend":[{"year":2018,"count":1}],"oa_status":"closed","license":"https://www.karger.com/Services/SiteLicenses","oa_locations":[{"url":"https://www.karger.com/Article/Pdf/22536","host_type":"publisher"},{"url":"https://doi.org/10.1159/000022536","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11014964","host_type":"repository"},{"url":"http://content.karger.com/produktedb/produkte.asp?typ=fulltext&file=hae30139","host_type":"repository"}],"fields_of_study":["Platelet Disorders and Treatments","Blood groups and transfusion","Heparin-Induced Thrombocytopenia and Thrombosis","Chemistry","Medicine","Biology","Animals","Antibodies, Monoclonal","Antibody Specificity","Binding, Competitive","Crotalid Venoms","Enzyme-Linked Immunosorbent Assay","Humans","Mice","Mice, Inbred BALB C","Platelet Adhesiveness","Platelet Aggregation","Platelet Glycoprotein GPIb-IX Complex","Protein Binding","Ristocetin","Stress, Mechanical","von Willebrand Factor"],"mesh_terms":["Animals","Antibodies, Monoclonal","Antibody Specificity","Binding, Competitive","Crotalid Venoms","Enzyme-Linked Immunosorbent Assay","Humans","Mice, Inbred BALB C","Platelet Adhesiveness","Platelet Aggregation","Protein Binding","Ristocetin","Stress, Mechanical","von Willebrand Factor","Platelet Glycoprotein GPIb-IX Complex","Mice"],"keywords":["Platelet membrane glycoprotein","Von Willebrand factor","Glycoprotein Ib","Ristocetin","Platelet","Platelet Glycoprotein GPIb-IX Complex","Chemistry","Glycoprotein","Monoclonal antibody","Molecular biology","In vivo","Von Willebrand disease","Biophysics","Antibody","Biochemistry","Immunology","Medicine","Biology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"No poverty"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T19:23:37.043760Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}