{"doi":"10.1158/1078-0432.ccr-05-0683","title":"Novel Genes Associated with Malignant Melanoma but not Benign Melanocytic Lesions","abstract":"<h4>Purpose</h4>Cutaneous melanoma is a common, aggressive cancer with increasing incidence. The identification of melanoma-specific deregulated genes could provide molecular markers for lymph node staging assays and further insight into melanoma tumorigenesis.<h4>Experimental design</h4>Total RNA isolated from 45 primary melanoma, 18 benign skin nevi, and 7 normal skin tissue specimens were analyzed on an Affymetrix Hu133A microarray containing 22,000 probe sets.<h4>Results</h4>Hierarchical clustering revealed a distinct separation of the melanoma samples from the benign and normal specimens. Novel genes associated with malignant melanoma were identified. Differential gene expression of two melanoma-specific genes, PLAB and L1CAM, were tested by a one-step quantitative reverse transcription-PCR assay on primary malignant melanoma, benign nevi, and normal skin samples, as well as on malignant melanoma lymph node metastasis and melanoma-free lymph nodes. The performance of the markers was compared with conventional melanoma markers such as tyrosinase, gp100, and MART1.<h4>Conclusion</h4>Our study systematically identified novel melanoma-specific genes and showed the feasibility of using a combination of PLAB and L1CAM in a reverse transcription-PCR assay to differentiate clinically relevant samples containing benign or malignant melanocytes.","journal":"Clinical Cancer Research","year":2005,"id":10424,"datarank":0.9406482647787451,"base_score":6.270988431858299,"endowment":6.270988431858299,"self_citation_contribution":0.9406482647787451,"citation_network_contribution":0.0,"self_endowment_contribution":0.9406482647787451,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":528,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0946,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2005-10-15","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":86913,"name":"Abhijit Mazumder","orcid":"0000-0001-9944-5306","position":1,"is_corresponding":false},{"id":86914,"name":"Jack X. Yu","orcid":null,"position":2,"is_corresponding":false},{"id":86915,"name":"Thomas Briggs","orcid":null,"position":3,"is_corresponding":false},{"id":86916,"name":"Yuqiu Jiang","orcid":null,"position":4,"is_corresponding":false},{"id":86917,"name":"John Backus","orcid":null,"position":5,"is_corresponding":false},{"id":86918,"name":"David Atkins","orcid":null,"position":6,"is_corresponding":false},{"id":86919,"name":"Yixin Wang","orcid":"0000-0003-4324-2408","position":7,"is_corresponding":false},{"id":86920,"name":"T. Briggs","orcid":null,"position":8,"is_corresponding":false},{"id":86912,"name":"Dmitri Talantov","orcid":null,"position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}