{"doi":"10.1158/0008-5472.c.6497882","title":"Data from DNA Methyltransferase 1 and 3B Activate <i>BAG-1</i> Expression via Recruitment of CTCFL/BORIS and Modulation of Promoter Histone Methylation","abstract":"<div>Abstract<p>In a previous genomic analysis, using somatic methyltransferase (<i>DNMT</i>) knockout cells, we showed that hypomethylation decreased the expression of as many genes as were observed to increase, suggesting a previously unknown mechanism for epigenetic regulation. To address this idea, the expression of the <i>BAG</i> family genes was used as a model. These genes were used because their expression was decreased in DNMT1<sup>−/−</sup>, DNMT3B<sup>−/−</sup>, and double knockout cells and increased in <i>DNMT1</i>-overexpressing and <i>DNMT3B</i>-overexpressing cells. Chromatin immunoprecipitation analysis of the <i>BAG-1</i> promoter in <i>DNMT1</i>-overexpressing or <i>DNMT3B</i>-overexpressing cells showed a permissive dimethyl-H3-K4/dimethyl-H3-K9 chromatin status associated with DNA-binding of CTCFL/BORIS, as well as increased <i>BAG-1</i> expression. In contrast, a nonpermissive dimethyl-H3-K4/dimethyl-H3-K9 chromatin status was associated with CTCF DNA-binding and decreased <i>BAG-1</i> expression in the single and double <i>DNMT</i> knockout cells. <i>BORIS</i> short hairpin RNA knockdown decreased both promoter DNA-binding, as well as <i>BAG-1</i> expression, and changed the dimethyl-H3-K4/dimethyl-H3-K9 ratio to that characteristic of a nonpermissive chromatin state. These results suggest that <i>DNMT1</i> and <i>DNMT3B</i> regulate <i>BAG-1</i> expression via insulator protein DNA-binding and chromatin dynamics by regulating histone dimethylation. [Cancer Res 2008;68(8):2726–35]</p></div>","journal":null,"year":2023,"id":404915,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9432,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":590580,"name":"Lei Huang","orcid":"0000-0002-0568-0645","position":1,"is_corresponding":false},{"id":1183644,"name":"Phuongmai Nguyen","orcid":null,"position":2,"is_corresponding":false},{"id":1183281,"name":"Kheem S. Bisht","orcid":"0000-0001-9595-738X","position":3,"is_corresponding":false},{"id":1183282,"name":"Gil Bar‐Sela","orcid":"0000-0001-6579-1841","position":4,"is_corresponding":false},{"id":630608,"name":"Allen S. Ho","orcid":"0000-0001-5423-509X","position":5,"is_corresponding":false},{"id":1183645,"name":"C. Matthew Bradbury","orcid":null,"position":6,"is_corresponding":false},{"id":1183646,"name":"Wenqiang Yu","orcid":null,"position":7,"is_corresponding":false},{"id":60691,"name":"Hengmi Cui","orcid":"0000-0001-5139-5370","position":8,"is_corresponding":false},{"id":1183283,"name":"Sun-Min Lee","orcid":"0000-0001-8435-2430","position":9,"is_corresponding":false},{"id":232026,"name":"Jane B. Trepel","orcid":"0000-0002-7681-6554","position":10,"is_corresponding":false},{"id":29384,"name":"Andrew P. Feinberg","orcid":"0000-0002-8364-1991","position":11,"is_corresponding":false},{"id":381615,"name":"David Gius","orcid":"0000-0001-9647-3571","position":12,"is_corresponding":false},{"id":1183643,"name":"Lunching Sun","orcid":null,"position":0,"is_corresponding":true}],"reference_count":31,"raw_metadata":null,"created_at":"2026-07-19T01:20:49.237119Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}