{"doi":"10.1158/0008-5472.c.6497355","title":"Data from CD8<sup>+</sup> T-Cell Responses against Hemoglobin-β Prevent Solid Tumor Growth","abstract":"<div>Abstract<p>Bone marrow–derived dendritic cells engineered using recombinant adenovirus to secrete high levels of IL-12p70 dramatically inhibited the growth of established CMS4 sarcomas in BALB/c mice after intratumoral administration. An analysis of splenic CD8<sup>+</sup> T cells in regressor mice revealed a strong, complex reactivity pattern against high-performance liquid chromatography (HPLC)–resolved peptides isolated by acid elution from single-cell suspensions of surgically resected CMS4 lesions. Mass spectrometry analyses defined two major overlapping peptide species that derive from the murine hemoglobin-β (HBB) protein within the most stimulatory HPLC fractions. Although cultured CMS4 tumor cells failed to express HBB mRNA based on reverse transcription-PCR analyses, prophylactic vaccination of BALB/c mice with vaccines containing HBB peptides promoted specific CD8<sup>+</sup> T-cell responses that protected mice against a subsequent challenge with CMS4 or unrelated syngeneic (HBB<sup>neg</sup>) tumors of divergent histology (sarcoma, carcinomas of the breast or colon). <i>In situ</i> imaging suggested that vaccines limit or destabilize tumor-associated vascular structures, potentially by promoting immunity against HBB+ vascular pericytes. Importantly, there were no untoward effects of vaccination with the HBB peptide on peripheral RBC numbers, RBC hemoglobin content, or vascular structures in the brain or eye. [Cancer Res 2008;68(19):8076–84]</p></div>","journal":null,"year":2023,"id":404316,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9572,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1182674,"name":"Zhao Xi","orcid":null,"position":1,"is_corresponding":false},{"id":624128,"name":"Jennifer L. Taylor","orcid":"0000-0002-4785-5933","position":2,"is_corresponding":false},{"id":647929,"name":"Louis J. Sparvero","orcid":"0000-0001-6347-8448","position":3,"is_corresponding":false},{"id":235148,"name":"Andrew A. Amoscato","orcid":"0000-0002-1340-9150","position":4,"is_corresponding":false},{"id":1182675,"name":"Sean Alber","orcid":null,"position":5,"is_corresponding":false},{"id":279286,"name":"Simon C. Watkins","orcid":"0000-0003-4092-1552","position":6,"is_corresponding":false},{"id":1182676,"name":"Angela D. Pardee","orcid":null,"position":7,"is_corresponding":false},{"id":1182227,"name":"Amy Wesa","orcid":"0000-0002-4917-5165","position":8,"is_corresponding":false},{"id":311651,"name":"Walter J. Storkus","orcid":"0000-0001-8961-4444","position":9,"is_corresponding":false},{"id":1182673,"name":"Hideo Komita","orcid":null,"position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:20:44.591715Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}