{"doi":"10.1155/2017/5296516","title":"p21-Activated Kinase 4 Promotes Intimal Hyperplasia and Vascular Smooth Muscle Cells Proliferation during Superficial Femoral Artery Restenosis after Angioplasty","abstract":"<jats:p>The aim of this study is to explore the function of p21-activated kinase 4 (PAK4) in intimal hyperplasia (IH) and vascular smooth muscle cells (VSMCs) proliferation. We choose vascular samples from patients undergoing angioplasty in superficial femoral artery (SFA) as the experimental group and vascular samples from donors without clinical SFA restenosis as the control group, respectively. We draw from the results that both levels of mRNA and protein of PAK4 in the experimental group increased dramatically compared with the control group. IH arose from angioplasty of SFA. Moreover, overexpression of PAK4 dramatically contributed to cell proliferation of VSMCs and promoted cell cycle progression from G0/G1 phase (<mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"M1\"><mml:mn fontstyle=\"italic\">71.12</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle=\"italic\">0.69</mml:mn></mml:math>% versus <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"M2\"><mml:mn fontstyle=\"italic\">58.77</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle=\"italic\">0.77</mml:mn></mml:math>%, <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"M3\"><mml:mi>P</mml:mi><mml:mo>&lt;</mml:mo><mml:mn fontstyle=\"italic\">0.001</mml:mn></mml:math>) into S phase (<mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"M4\"><mml:mn fontstyle=\"italic\">23.99</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle=\"italic\">0.21</mml:mn></mml:math>% versus <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"M5\"><mml:mn fontstyle=\"italic\">31.35</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle=\"italic\">0.33</mml:mn></mml:math>%, <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"M6\"><mml:mi>P</mml:mi><mml:mo>&lt;</mml:mo><mml:mn fontstyle=\"italic\">0.001</mml:mn></mml:math>). Besides, PAK4 downregulated the level of p21 and enhanced the activity of Akt as well. And we conclude that PAK4 acts as a regulator of cell cycle progression of VSMC by mediating Akt signaling and controlling p21 levels, which further modulate IH and VSMCs’ proliferation.</jats:p>","journal":"BioMed Research International","year":2017,"id":685929,"datarank":0.38474240361923057,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.0,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1792081,"name":"Xianli Duan","orcid":null,"position":1,"is_corresponding":false},{"id":806629,"name":"Jian Dong","orcid":"0000-0002-4661-8901","position":2,"is_corresponding":false},{"id":1792084,"name":"Qingsheng Lu","orcid":null,"position":3,"is_corresponding":false},{"id":88515,"name":"Jian Zhou","orcid":"0000-0002-9917-5622","position":4,"is_corresponding":false},{"id":1317923,"name":"Zhiqing Zhao","orcid":"0000-0002-1108-6271","position":5,"is_corresponding":false},{"id":1792086,"name":"Junmin Bao","orcid":"0000-0002-5132-7500","position":6,"is_corresponding":false},{"id":1792087,"name":"Zaiping Jing","orcid":"0000-0002-7022-4953","position":7,"is_corresponding":false},{"id":1792079,"name":"Liangxi Yuan","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"p21-Activated Kinase 4 Promotes Intimal Hyperplasia and Vascular Smooth Muscle Cells Proliferation during Superficial Femoral Artery Restenosis after Angioplasty","abstract":"<jats:p>The aim of this study is to explore the function of p21-activated kinase 4 (PAK4) in intimal hyperplasia (IH) and vascular smooth muscle cells (VSMCs) proliferation. We choose vascular samples from patients undergoing angioplasty in superficial femoral artery (SFA) as the experimental group and vascular samples from donors without clinical SFA restenosis as the control group, respectively. We draw from the results that both levels of mRNA and protein of PAK4 in the experimental group increased dramatically compared with the control group. IH arose from angioplasty of SFA. Moreover, overexpression of PAK4 dramatically contributed to cell proliferation of VSMCs and promoted cell cycle progression from G0/G1 phase (<mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"M1\"><mml:mn fontstyle=\"italic\">71.12</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle=\"italic\">0.69</mml:mn></mml:math>% versus <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"M2\"><mml:mn fontstyle=\"italic\">58.77</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle=\"italic\">0.77</mml:mn></mml:math>%, <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"M3\"><mml:mi>P</mml:mi><mml:mo>&lt;</mml:mo><mml:mn fontstyle=\"italic\">0.001</mml:mn></mml:math>) into S phase (<mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"M4\"><mml:mn fontstyle=\"italic\">23.99</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle=\"italic\">0.21</mml:mn></mml:math>% versus <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"M5\"><mml:mn fontstyle=\"italic\">31.35</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle=\"italic\">0.33</mml:mn></mml:math>%, <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"M6\"><mml:mi>P</mml:mi><mml:mo>&lt;</mml:mo><mml:mn fontstyle=\"italic\">0.001</mml:mn></mml:math>). Besides, PAK4 downregulated the level of p21 and enhanced the activity of Akt as well. And we conclude that PAK4 acts as a regulator of cell cycle progression of VSMC by mediating Akt signaling and controlling p21 levels, which further modulate IH and VSMCs’ proliferation.</jats:p>","is_dataset_classified":null,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28706947","pmcid":"PMC5494543","openalex_id":"https://openalex.org/W2686055919","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"81500390","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"81370441","title":null}],"total_grants":2,"fwci":0.7089,"citation_percentile":0.71307254,"influential_citations":0,"citation_trend":[{"year":2017,"count":1},{"year":2018,"count":1},{"year":2020,"count":1},{"year":2021,"count":3},{"year":2022,"count":4},{"year":2023,"count":2}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://downloads.hindawi.com/journals/bmri/2017/5296516.pdf","host_type":"journal"},{"url":"http://downloads.hindawi.com/journals/bmri/2017/5296516.pdf","host_type":"publisher"},{"url":"http://downloads.hindawi.com/journals/bmri/2017/5296516.xml","host_type":"publisher"},{"url":"https://doi.org/10.1155/2017/5296516","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28706947","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5494543","host_type":"repository"},{"url":"https://doaj.org/article/066d9f5630da4aad89ce6c9d8e06905d","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5494543","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5494543?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Lipoproteins and Cardiovascular Health","Inflammatory mediators and NSAID effects","Coronary Interventions and Diagnostics"],"mesh_terms":["Aged","Animals","Cell Cycle","Female","Femoral Artery","Gene Expression Regulation","Humans","Hyperplasia","Male","Muscle, Smooth, Vascular","Signal Transduction","Angioplasty","Tunica Intima","Myocytes, Smooth Muscle","Cell Proliferation","Cyclin-Dependent Kinase Inhibitor p21","p21-Activated Kinases"],"keywords":["Restenosis","Intimal hyperplasia","Vascular smooth muscle","Angioplasty","Medicine","Femoral artery","Cell cycle","Cell growth","Hyperplasia","Protein kinase B","Neointimal hyperplasia","Cancer research","Artery","Internal medicine","Endocrinology","Smooth muscle","Phosphorylation","Chemistry","Cell biology","Biology","Stent","Cancer","Biochemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T18:04:06.749266Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}