{"doi":"10.1155/2016/6397820","title":"Decellularized Extracellular Matrix as an <i>In Vitro</i> Model to Study the Comprehensive Roles of the ECM in Stem Cell Differentiation","abstract":"<jats:p>Stem cells are a promising cell source for regenerative medicine. Stem cell differentiation must be regulated for applications in regenerative medicine. Stem cells are surrounded by extracellular matrix (ECM) <jats:italic>in vivo</jats:italic>. The ECM is composed of many types of proteins and glycosaminoglycans that assemble into a complex structure. The assembly of ECM molecules influences stem cell differentiation through orchestrated intracellular signaling activated by many ECM molecules. Therefore, it is important to understand the comprehensive role of the ECM in stem cell differentiation as well as the functions of the individual ECM molecules. Decellularized ECM is a useful <jats:italic>in vitro</jats:italic> model for studying the comprehensive roles of ECM because it retains a native‐like structure and composition. Decellularized ECM can be obtained from <jats:italic>in vivo</jats:italic> tissue ECM or ECM fabricated by cells cultured <jats:italic>in vitro</jats:italic>. It is important to select the correct decellularized ECM because each type has different properties. In this review, tissue‐derived and cell‐derived decellularized ECMs are compared as <jats:italic>in vitro</jats:italic> ECM models to examine the comprehensive roles of the ECM in stem cell differentiation. We also summarize recent studies using decellularized ECM to determine the comprehensive roles of the ECM in stem cell differentiation.</jats:p>","journal":"Stem Cells International","year":2016,"id":593803,"datarank":0.7838620010569803,"base_score":5.225746673713202,"endowment":5.225746673713202,"self_citation_contribution":0.7838620010569803,"citation_network_contribution":0.0,"self_endowment_contribution":0.7838620010569803,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":185,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":2,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1314260,"name":"Guoping Chen","orcid":"0000-0001-6753-3678","position":1,"is_corresponding":false},{"id":1519848,"name":"Chiho Endo","orcid":null,"position":2,"is_corresponding":false},{"id":1519849,"name":"Hiroka Maruyama","orcid":null,"position":3,"is_corresponding":false},{"id":1519851,"name":"Miyuki Wakui","orcid":null,"position":4,"is_corresponding":false},{"id":1519852,"name":"Eri Nemoto","orcid":null,"position":5,"is_corresponding":false},{"id":1314259,"name":"Naoki Kawazoe","orcid":"0000-0003-3916-0709","position":6,"is_corresponding":false},{"id":571505,"name":"Masaru Tanaka","orcid":"0000-0003-4383-4024","position":7,"is_corresponding":false},{"id":1519847,"name":"Takashi Hoshiba","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Decellularized Extracellular Matrix as an <i>In Vitro</i> Model to Study the Comprehensive Roles of the ECM in Stem Cell Differentiation","abstract":"<jats:p>Stem cells are a promising cell source for regenerative medicine. Stem cell differentiation must be regulated for applications in regenerative medicine. Stem cells are surrounded by extracellular matrix (ECM) <jats:italic>in vivo</jats:italic>. The ECM is composed of many types of proteins and glycosaminoglycans that assemble into a complex structure. The assembly of ECM molecules influences stem cell differentiation through orchestrated intracellular signaling activated by many ECM molecules. Therefore, it is important to understand the comprehensive role of the ECM in stem cell differentiation as well as the functions of the individual ECM molecules. Decellularized ECM is a useful <jats:italic>in vitro</jats:italic> model for studying the comprehensive roles of ECM because it retains a native‐like structure and composition. Decellularized ECM can be obtained from <jats:italic>in vivo</jats:italic> tissue ECM or ECM fabricated by cells cultured <jats:italic>in vitro</jats:italic>. It is important to select the correct decellularized ECM because each type has different properties. In this review, tissue‐derived and cell‐derived decellularized ECMs are compared as <jats:italic>in vitro</jats:italic> ECM models to examine the comprehensive roles of the ECM in stem cell differentiation. We also summarize recent studies using decellularized ECM to determine the comprehensive roles of the ECM in stem cell differentiation.</jats:p>","is_dataset_classified":null,"base_score":5.225746673713202,"endowment":5.225746673713202,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26770210","pmcid":"PMC4684892","openalex_id":"https://openalex.org/W1497757154","authors":[],"funders":[{"funder_name":"Ministry of Education, Culture, Sports, Science, and Technology","grant_id":"2672016","title":null}],"total_grants":1,"fwci":11.6564,"citation_percentile":0.99061299,"influential_citations":0,"citation_trend":[{"year":2016,"count":6},{"year":2017,"count":17},{"year":2018,"count":18},{"year":2019,"count":16},{"year":2020,"count":26},{"year":2021,"count":17},{"year":2022,"count":23},{"year":2023,"count":15},{"year":2024,"count":23},{"year":2025,"count":22},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"http://downloads.hindawi.com/journals/sci/2016/6397820.pdf","host_type":"journal"},{"url":"http://downloads.hindawi.com/journals/sci/2016/6397820.pdf","host_type":"publisher"},{"url":"http://downloads.hindawi.com/journals/sci/2016/6397820.xml","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1155/2016/6397820","host_type":"publisher"},{"url":"https://doi.org/10.1155/2016/6397820","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26770210","host_type":"repository"},{"url":"https://doaj.org/article/9fbfaca74c98462f9eec28d244349c17","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4684892","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4684892","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4684892?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Tissue Engineering and Regenerative Medicine","Electrospun Nanofibers in Biomedical Applications","3D Printing in Biomedical Research"],"mesh_terms":[],"keywords":["Decellularization","Extracellular matrix","Cell biology","Stem cell","Regenerative medicine","Cellular differentiation","Tissue engineering","Chemistry","Biology","Biochemistry","Genetics"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.26632315.v1","title":"Additional file 1 of Bioengineered skin constructs based on mesenchymal stromal cells and acellular dermal matrix exposed to inflammatory microenvironment releasing growth factors involved in skin repair","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.26632315","title":"Additional file 1 of Bioengineered skin constructs based on mesenchymal stromal cells and acellular dermal matrix exposed to inflammatory microenvironment releasing growth factors involved in skin repair","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T12:18:03.307700Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}