{"doi":"10.1155/2014/849743","title":"New Strategies for Evaluation and Analysis of SELEX Experiments","abstract":"<jats:p>Aptamers are an interesting alternative to antibodies in pharmaceutics and biosensorics, because they are able to bind to a multitude of possible target molecules with high affinity. Therefore the process of finding such aptamers, which is commonly a SELEX screening process, becomes crucial. The standard SELEX procedure schedules the validation of certain found aptamers via binding experiments, which is not leading to any detailed specification of the aptamer enrichment during the screening. For the purpose of advanced analysis of the accrued enrichment within the SELEX library we used sequence information gathered by next generation sequencing techniques in addition to the standard SELEX procedure. As sequence motifs are one possibility of enrichment description, the need of finding those recurring sequence motifs corresponding to substructures within the aptamers, which are characteristically fitted to specific binding sites of the target, arises. In this paper a motif search algorithm is presented, which helps to describe the aptamers enrichment in more detail. The extensive characterization of target and binding aptamers may later reveal a functional connection between these molecules, which can be modeled and used to optimize future SELEX runs in case of the generation of target-specific starting libraries.</jats:p>","journal":"BioMed Research International","year":2014,"id":13809,"datarank":1.2622484211384657,"base_score":3.295836866004329,"endowment":3.295836866004329,"self_citation_contribution":0.4943755299006494,"citation_network_contribution":0.7678728912378162,"self_endowment_contribution":0.4943755299006494,"citer_contribution":0.7678728912378162,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":25,"citers_with_citation_signal":21,"citers_with_endowment":21,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":111747,"name":"Elke Boschke","orcid":null,"position":1,"is_corresponding":false},{"id":111748,"name":"Dirk Labudde","orcid":null,"position":2,"is_corresponding":false},{"id":111746,"name":"Rico Beier","orcid":"0000-0003-3353-5039","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.295836866004329,"endowment":3.295836866004329,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24779017","pmcid":"PMC3977542","openalex_id":"https://openalex.org/W1965088961","authors":[],"funders":[{"funder_name":"European Social Fund","grant_id":"","title":null},{"funder_name":"Free State of Saxony","grant_id":"","title":null}],"total_grants":2,"fwci":1.7071,"citation_percentile":0.83314348,"influential_citations":1,"citation_trend":[{"year":2014,"count":2},{"year":2015,"count":3},{"year":2016,"count":4},{"year":2017,"count":3},{"year":2018,"count":3},{"year":2019,"count":3},{"year":2020,"count":2},{"year":2022,"count":5},{"year":2025,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://downloads.hindawi.com/journals/bmri/2014/849743.pdf","host_type":"journal"},{"url":"https://downloads.hindawi.com/journals/bmri/2014/849743.pdf","host_type":"GOLD"},{"url":"https://downloads.hindawi.com/journals/bmri/2014/849743.pdf","host_type":"publisher"},{"url":"http://downloads.hindawi.com/journals/bmri/2014/849743.pdf","host_type":"publisher"},{"url":"http://downloads.hindawi.com/journals/bmri/2014/849743.xml","host_type":"publisher"},{"url":"https://doi.org/10.1155/2014/849743","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24779017","host_type":"repository"},{"url":"https://doaj.org/article/70fbb25155584ee3b405b43d1949596e","host_type":"repository"},{"url":"http://europepmc.org/articles/PMC3977542","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3977542","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3977542","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3977542?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Advanced biosensing and bioanalysis techniques","Monoclonal and Polyclonal Antibodies Research","RNA and protein synthesis mechanisms","Biology","Medicine","Chemistry","Aptamers, Nucleotide","Binding Sites","Capsid Proteins","High-Throughput Nucleotide Sequencing","Norovirus","Nucleic Acid Conformation","Nucleotide Motifs","SELEX Aptamer Technique"],"mesh_terms":["Binding Sites","Nucleic Acid Conformation","Norovirus","Capsid Proteins","SELEX Aptamer Technique","Aptamers, Nucleotide","High-Throughput Nucleotide Sequencing","Nucleotide Motifs"],"keywords":["Aptamer","Systematic evolution of ligands by exponential enrichment","Computational biology","Sequence (biology)","Computer science","Biology","Genetics","Gene","RNA"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-05-31T17:32:01.140569Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}