{"doi":"10.1155/2012/105891","title":"Mouse Ficolin B Has an Ability to Form Complexes with Mannose-Binding Lectin-Associated Serine Proteases and Activate Complement through the Lectin Pathway","abstract":"Ficolins are thought to be pathogen-associated-molecular-pattern-(PAMP-) recognition molecules that function to support innate immunity. Like mannose-binding lectins (MBLs), most mammalian ficolins form complexes with MBL-associated serine proteases (MASPs), leading to complement activationviathe lectin pathway. However, the ability of murine ficolin B, a homologue of human M-ficolin, to perform this function is still controversial. The results of the present study show that ficolin B in mouse bone marrow is an oligomeric protein. Ficolin B, pulled down using GlcNAc-agarose, contained very low, but detectable, amounts of MASP-2 and small MBL-associated protein (sMAP) and showed detectable C4-deposition activity on immobilizedN-acetylglucosamine. These biochemical features of ficolin B were confirmed using recombinant mouse ficolin B produced in CHO cells. Taken together, these results suggest that like other mammalian homologues, murine ficolin B has an ability to exert its functionviathe lectin pathway.","journal":"Journal of Biomedicine and Biotechnology","year":2012,"id":18316,"datarank":0.9756268494947107,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":0.526267008461612,"self_endowment_contribution":0.4493598410330987,"citer_contribution":0.526267008461612,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":14,"citers_with_citation_signal":13,"citers_with_endowment":13,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":123116,"name":"Daisuke Iwaki","orcid":null,"position":1,"is_corresponding":false},{"id":123118,"name":"Yumi Ishida","orcid":null,"position":2,"is_corresponding":false},{"id":123115,"name":"Minoru Takahashi","orcid":"0000-0001-7625-2448","position":3,"is_corresponding":false},{"id":122927,"name":"Misao Matsushita","orcid":null,"position":4,"is_corresponding":false},{"id":122928,"name":"Teizo Fujita","orcid":null,"position":5,"is_corresponding":false},{"id":123106,"name":"Yuichi Endo","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.995732273553991,"endowment":2.995732273553991,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22523468","pmcid":"PMC3306798","openalex_id":"https://openalex.org/W2041992276","authors":[],"funders":[],"total_grants":0,"fwci":1.1547,"citation_percentile":0.76780443,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":4},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":2},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2025,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://downloads.hindawi.com/journals/bmri/2012/105891.pdf","host_type":"journal"},{"url":"https://downloads.hindawi.com/journals/bmri/2012/105891.pdf","host_type":"GOLD"},{"url":"https://downloads.hindawi.com/journals/bmri/2012/105891.pdf","host_type":"publisher"},{"url":"http://downloads.hindawi.com/journals/bmri/2012/105891.pdf","host_type":"publisher"},{"url":"http://downloads.hindawi.com/journals/bmri/2012/105891.xml","host_type":"publisher"},{"url":"https://doi.org/10.1155/2012/105891","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22523468","host_type":"repository"},{"url":"https://doaj.org/article/2ea1d76a90af431d8a84a5c90e3c6f1c","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3306798","host_type":"repository"},{"url":"http://www.hindawi.com/GetPDF.aspx?doi=10.1155/2012/105891","host_type":"hindawi.com"},{"url":"https://europepmc.org/articles/PMC3306798","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3306798?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Complement system in diseases","Invertebrate Immune Response Mechanisms","Trypanosoma species research and implications","Biology","Medicine","Acetylglucosamine","Animals","Blotting, Western","Bone Marrow","Complement C4","Complement Pathway, Mannose-Binding Lectin","Humans","Lectins","Mannose-Binding Protein-Associated Serine Proteases","Mice","Recombinant Proteins","Ficolins"],"mesh_terms":["Ficolins","Acetylglucosamine","Animals","Bone Marrow","Complement C4","Humans","Recombinant Proteins","Blotting, Western","Lectins","Complement Pathway, Mannose-Binding Lectin","Mannose-Binding Protein-Associated Serine Proteases","Mice"],"keywords":["Ficolin","Lectin pathway","Mannan-binding lectin","MASP1","Lectin","Proteases","Complement system","Serine","Mannose","Biochemistry","C-type lectin","Chemistry","Complement component 2","Classical complement pathway","Serine protease","Biology","Immunology","Antibody","Enzyme","Protease"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-03T20:55:33.920165Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}