{"doi":"10.1152/ajpcell.1994.267.4.c1112","title":"MCP-1-stimulated monocyte attachment to laminin is mediated by beta 2-integrins","abstract":"<jats:p> Migration of monocytes to sites of inflammation involves a series of attachments and detachments to extracellular matrix proteins. We examined the capacity of a chemokine, monocyte chemoattractant protein-1 (MCP-1), to regulate attachment of human monocytes to laminin, collagen I, collagen IV, or fibronectin. MCP-1 increased monocyte attachment to laminin in a dose- and time-dependent manner and stimulated a lesser increase to the other matrix proteins. Function-blocking monoclonal antibodies (MAbs) to the integrin beta 2-subunit (CD18), including Fab' fragments and alpha M (CD11b) blocked &gt; 70% of attachment, whereas MAbs to the beta 1-integrin subunit reduced attachment by &lt; 30%. This suggests that the CD11b/CD18 integrin is the predominant molecule involved in adhesion of MCP-1-stimulated monocytes to laminin. The association of CD11b with F-actin illustrated by confocal microscopy further supports this concept. In contrast, when monocytes were stimulated with the beta 1-stimulatory MAb TS2/16, monocyte adhesion to laminin occurred through beta 1-integrins. Thus MCP-1 can stimulate monocyte attachment to laminin, and this process is mediated through beta 2-integrins, principally CD11b/CD18. </jats:p>","journal":"American Journal of Physiology-Cell Physiology","year":1994,"id":619067,"datarank":0.5709993734655481,"base_score":3.8066624897703196,"endowment":3.8066624897703196,"self_citation_contribution":0.5709993734655481,"citation_network_contribution":0.0,"self_endowment_contribution":0.5709993734655481,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":44,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1597454,"name":"J. F. Zhu","orcid":null,"position":1,"is_corresponding":false},{"id":1597455,"name":"F. W. Luscinskas","orcid":null,"position":2,"is_corresponding":false},{"id":1597458,"name":"D. T. Graves","orcid":null,"position":3,"is_corresponding":false},{"id":1479412,"name":"Y. Jiang","orcid":"0000-0002-4898-0787","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"MCP-1-stimulated monocyte attachment to laminin is mediated by beta 2-integrins","abstract":"<jats:p> Migration of monocytes to sites of inflammation involves a series of attachments and detachments to extracellular matrix proteins. We examined the capacity of a chemokine, monocyte chemoattractant protein-1 (MCP-1), to regulate attachment of human monocytes to laminin, collagen I, collagen IV, or fibronectin. MCP-1 increased monocyte attachment to laminin in a dose- and time-dependent manner and stimulated a lesser increase to the other matrix proteins. Function-blocking monoclonal antibodies (MAbs) to the integrin beta 2-subunit (CD18), including Fab' fragments and alpha M (CD11b) blocked &gt; 70% of attachment, whereas MAbs to the beta 1-integrin subunit reduced attachment by &lt; 30%. This suggests that the CD11b/CD18 integrin is the predominant molecule involved in adhesion of MCP-1-stimulated monocytes to laminin. The association of CD11b with F-actin illustrated by confocal microscopy further supports this concept. In contrast, when monocytes were stimulated with the beta 1-stimulatory MAb TS2/16, monocyte adhesion to laminin occurred through beta 1-integrins. Thus MCP-1 can stimulate monocyte attachment to laminin, and this process is mediated through beta 2-integrins, principally CD11b/CD18. </jats:p>","is_dataset_classified":null,"base_score":3.8066624897703196,"endowment":3.8066624897703196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7943274","pmcid":null,"openalex_id":"https://openalex.org/W2408534315","authors":[],"funders":[{"funder_name":"NIDCR NIH HHS","grant_id":"DE-09581","title":null},{"funder_name":"NIDCR NIH HHS","grant_id":"DE-07559","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"HL-36028","title":null}],"total_grants":3,"fwci":0.8613,"citation_percentile":0.72659817,"influential_citations":0,"citation_trend":[{"year":2014,"count":2},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://journals.physiology.org/doi/pdf/10.1152/ajpcell.1994.267.4.C1112","host_type":"publisher"},{"url":"https://doi.org/10.1152/ajpcell.1994.267.4.c1112","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7943274","host_type":"repository"}],"fields_of_study":["Cell Adhesion Molecules Research","Immune Response and Inflammation","Chemokine receptors and signaling"],"mesh_terms":["Antibodies, Monoclonal","Cell Adhesion","Chemotactic Factors","Humans","Laminin","Monocytes","Integrins","Cytokines","Extracellular Matrix Proteins","Microscopy, Confocal","Chemokine CCL2"],"keywords":["Integrin alpha M","Integrin","CD18","Monocyte","Fibronectin","Laminin","Cell biology","Extracellular matrix","Chemistry","Molecular biology","Chemokine","Cell adhesion molecule","Cell adhesion","Chemotaxis","Adhesion","Biology","Immunology","Receptor","Biochemistry","Flow cytometry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T05:55:30.266719Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}