{"doi":"10.1152/ajpcell.1984.246.1.c141","title":"Phosphoinositide breakdown in blowfly salivary glands","abstract":"<jats:p> In blowfly salivary glands, 5-hydroxytryptamine stimulated a rapid and sustained loss of [3H]inositol, [32P]phosphatidylinositol, phosphatidylinositol 4-phosphate, and phosphatidylinositol 4,5-bisphosphate. There was a corresponding increase in labeled inositol phosphates. In the absence of Ca2+, 5-hydroxytryptamine stimulated a rapid but transient loss of labeled phosphatidylinositol 4,5-bisphosphate. By 5 min, the amount of labeled phosphatidylinositol 4,5-bisphosphate recovered to control values. The divalent ionophore A23187 stimulated loss of labeled phosphatidylinositol 4,5-bisphosphate and increased the amount of labeled phosphatidylinositol. In homogenates, Ca2+ stimulated phosphatidylinositol 4,5-bisphosphate breakdown but not phosphatidylinositol breakdown. These results suggest that hormone-stimulated breakdown of labeled phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate occurs through a phospholipase C and is relatively independent of extracellular Ca2+. There is also a Ca2+-activated conversion of phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol. </jats:p>","journal":"American Journal of Physiology-Cell Physiology","year":1984,"id":628126,"datarank":0.47670807455219194,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"self_citation_contribution":0.47670807455219194,"citation_network_contribution":0.0,"self_endowment_contribution":0.47670807455219194,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":23,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":73574,"name":"H. S. 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The divalent ionophore A23187 stimulated loss of labeled phosphatidylinositol 4,5-bisphosphate and increased the amount of labeled phosphatidylinositol. In homogenates, Ca2+ stimulated phosphatidylinositol 4,5-bisphosphate breakdown but not phosphatidylinositol breakdown. These results suggest that hormone-stimulated breakdown of labeled phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate occurs through a phospholipase C and is relatively independent of extracellular Ca2+. There is also a Ca2+-activated conversion of phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol. </jats:p>","is_dataset_classified":null,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"6320653","pmcid":null,"openalex_id":"https://openalex.org/W2415255352","authors":[],"funders":[{"funder_name":"NIADDK NIH HHS","grant_id":"AM-10149","title":null},{"funder_name":"NIADDK NIH HHS","grant_id":"AM-0657","title":null},{"funder_name":"NIADDK NIH HHS","grant_id":"AM-21470","title":null}],"total_grants":3,"fwci":2.4226,"citation_percentile":0.90151602,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://journals.physiology.org/doi/pdf/10.1152/ajpcell.1984.246.1.C141","host_type":"publisher"},{"url":"https://doi.org/10.1152/ajpcell.1984.246.1.c141","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/6320653","host_type":"repository"}],"fields_of_study":["Neurobiology and Insect Physiology Research","Protein Kinase Regulation and GTPase Signaling","Physiological and biochemical adaptations","Animals","Diptera","Inositol","Kinetics","Phosphatidylinositols","Phospholipids","Salivary Glands","Serotonin","Tritium"],"mesh_terms":["Animals","Diptera","Inositol","Kinetics","Phosphatidylinositols","Phospholipids","Salivary Glands","Serotonin","Tritium"],"keywords":["Phosphatidylinositol","Phosphatidylinositol 4,5-bisphosphate","Phospholipase C","Inositol","Inositol trisphosphate","Extracellular","Biochemistry","Chemistry","Biology","Cell biology","Enzyme","Signal transduction","Receptor"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T11:15:53.905319Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}