{"doi":"10.1152/ajpcell.00575.2024","title":"Alterations of the skeletal muscle nuclear proteome after acute exercise reveals a post-transcriptional influence.","abstract":"Exercise is firmly established as a key contributor to overall well-being and is frequently employed as a therapeutic approach to mitigate various health conditions. One pivotal aspect of the impact of exercise lies in the systemic transcriptional response, which underpins its beneficial adaptations. While extensive research has been devoted to understanding the transcriptional response to exercise, our knowledge of the protein constituents of nuclear processes accompanying gene expression in skeletal muscle remains largely elusive. We hypothesize that alterations in the nuclear proteome following exercise hold vital clues for comprehending exercise-induced transcriptional regulation and related nuclear functions. We first detail the successful isolation of skeletal muscle nuclei from C57BL/6 mice encapsulating 2,030 proteins linked to nuclear processes such as transcription, RNA processing, chromatin modifications, and nuclear transport. We then used this approach to isolate muscle nuclei in sedentary, immediately post-, 1-hour, and 4-hours after a 30-minute treadmill running session, to gain insight into the nuclear proteome after exercise. We found 54 proteins linked to mRNA splicing and nucleocytoplasmic transport, many of which were substantially reduced immediately post-exercise followed by a rapid increase 1- and 4-hours post-exercise. Super resolution microscopy experiments highlight localization changes in mRNA processing proteins post-exercise, further suggesting changes in nuclear transport dynamics. Our data provides important insight into changes in the nuclear proteome following exercise. In particular it highlights proteins contributing to mRNA processing and splicing in addition to transcriptional processes with exercise offering broader changes in mechanisms modulating the molecular response to acute exercise.","journal":"American Journal of Physiology-Cell Physiology","year":2025,"id":545967,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9599,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":483080,"name":"Xiping Zhang","orcid":"0000-0003-3202-451X","position":1,"is_corresponding":false},{"id":1021183,"name":"Mark Viggars","orcid":"0000-0002-0722-7051","position":2,"is_corresponding":false},{"id":235314,"name":"James Sanford","orcid":"0000-0001-7901-5579","position":3,"is_corresponding":false},{"id":555536,"name":"Zane W. Taylor","orcid":"0000-0002-5178-2570","position":4,"is_corresponding":false},{"id":589981,"name":"Joshua Hansen","orcid":"0000-0001-9237-2773","position":5,"is_corresponding":false},{"id":352735,"name":"Gérémy Clair","orcid":"0000-0003-0478-8520","position":6,"is_corresponding":false},{"id":814426,"name":"Collin M. Douglas","orcid":"0000-0002-7601-3508","position":7,"is_corresponding":false},{"id":1016988,"name":"Stuart J. Hesketh","orcid":"0000-0001-7855-2380","position":8,"is_corresponding":false},{"id":235317,"name":"Joshua Adkins","orcid":"0000-0003-0399-0700","position":9,"is_corresponding":false},{"id":291924,"name":"Karyn A. Esser","orcid":"0000-0002-5791-1441","position":10,"is_corresponding":false},{"id":435374,"name":"Ryan A. Martin","orcid":"0000-0002-5896-553X","position":0,"is_corresponding":true}],"reference_count":73,"raw_metadata":null,"created_at":"2026-07-19T02:53:27.751050Z","pmid":"40789650","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}