{"doi":"10.1139/o70-077","title":"Inhibition of mitochondrial and bacterial protein synthesis by chloramphenicol","abstract":"<jats:p> The structural requirements for the inhibition of protein synthesis in mitochondria and in bacterial extracts by chloramphenicol isomers and analogues are similar. D-threo-Chloramphenicol and its p-methylthio, p-methylsulfonyl, and p-sulfamoyl analogues equally inhibit protein synthesis in isolated rat-liver mitochondria and extracts of Escherichia coli B. Fifty percent inhibition is at 15 μM and 10 μM, respectively. Analogues with larger p-substituents on the phenyl ring or with an m-chloro group are less inhibitory in both systems. L-threo-Chloramphenicol and deacylated chloramphenicol do not inhibit mitochondrial protein synthesis; with a dichloroacetyl group replacing the acetyl group on chloramphenicol 50% inhibition is at 65 μM, and L-erythro-chloramphenicol is 2% as inhibitory as D-threo-chloramphenicol. The inhibition of protein synthesis in intact E. coli B is in the order: chloramphenicol &gt; p-methylthio &gt; p-methylsulfonyl &gt; p-sulfamoyl, 50% inhibition being at 4 μM for chloramphenicol. </jats:p>","journal":"Canadian Journal of Biochemistry","year":1970,"id":17758,"datarank":3.231008885563896,"base_score":3.8712010109078907,"endowment":3.8712010109078907,"self_citation_contribution":0.5806801516361837,"citation_network_contribution":2.6503287339277124,"self_endowment_contribution":0.5806801516361837,"citer_contribution":2.6503287339277124,"corpus_percentile":null,"corpus_rank":null,"citation_count":47,"citer_count":45,"citers_with_citation_signal":40,"citers_with_endowment":40,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":125918,"name":"K. B. Freeman","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.8712010109078907,"endowment":3.8712010109078907,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"4911451","pmcid":null,"openalex_id":"https://openalex.org/W2002414215","authors":[],"funders":[],"total_grants":0,"fwci":3.4258,"citation_percentile":0.91698324,"influential_citations":0,"citation_trend":[{"year":2020,"count":2},{"year":2024,"count":1},{"year":2025,"count":2}],"oa_status":"closed","license":"http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining","oa_locations":[{"url":"http://www.nrcresearchpress.com/doi/pdf/10.1139/o70-077","host_type":"publisher"},{"url":"https://doi.org/10.1139/o70-077","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/4911451","host_type":"repository"}],"fields_of_study":["Mitochondrial Function and Pathology","Metabolism and Genetic Disorders","RNA and protein synthesis mechanisms","Biology","Medicine","Chemistry","Animals","Bacterial Proteins","Carbon Isotopes","Chloramphenicol","Escherichia coli","Leucine","Lysine","Mitochondria, Liver","Protein Biosynthesis","Rats","Valine"],"mesh_terms":["Animals","Bacterial Proteins","Carbon Isotopes","Chloramphenicol","Escherichia coli","Leucine","Lysine","Mitochondria, Liver","Protein Biosynthesis","Valine","Rats"],"keywords":["Chloramphenicol","Escherichia coli","Protein biosynthesis","Mitochondrion","Chemistry","Biochemistry","Inhibitory postsynaptic potential","Antibiotics","Biology","Stereochemistry","Endocrinology"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-02T22:22:18.431355Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}