{"doi":"10.1139/m85-100","title":"Two types of xylanases of alkalophilic <i>Bacillus</i> sp. No. C-125","abstract":"<jats:p> One alkalophilic Bacillus sp. strain C-125 (FERM No. 7344) was isolated from soil. From this organism, two types of xylanases, designated xylanase A and xylanase N, were purified by an ammonium sulfate precipitation followed by Biogel P-30 gel filtration, DEAE-cellulose chromatography, and Sephadex G-75 gel filtration. The molecular weights of xylanase A and N were estimated as 43 000 and 16 000, respectively. Immunological experiments indicated that xylanase A and xylanase N were entirely different protein molecules. Xylanase N was most active at pH 6.0–7.0, but xylanase A had a very broad pH activity curve (pH 6–10) and was still active even at pH 12.0. The maximum hydrolysis of xylan by the enzymes was about 25%. Both enzymes split xylan and yielded xylobiose and higher oligosaccharides but could hydrolyze neither xylobiose nor xylotriose. Trans xylosidation activities were detected in both enzymes. </jats:p>","journal":"Canadian Journal of Microbiology","year":1985,"id":19694,"datarank":9.107151317444357,"base_score":4.624972813284271,"endowment":4.624972813284271,"self_citation_contribution":0.6937459219926407,"citation_network_contribution":8.413405395451717,"self_endowment_contribution":0.6937459219926407,"citer_contribution":8.413405395451717,"corpus_percentile":null,"corpus_rank":null,"citation_count":101,"citer_count":97,"citers_with_citation_signal":93,"citers_with_endowment":93,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":132568,"name":"T. Kudo","orcid":null,"position":1,"is_corresponding":false},{"id":132569,"name":"Y. Ikura","orcid":null,"position":2,"is_corresponding":false},{"id":132570,"name":"K. Horikoshi","orcid":null,"position":3,"is_corresponding":false},{"id":132567,"name":"H. Honda","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.624972813284271,"endowment":4.624972813284271,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21071399","pmcid":null,"openalex_id":"https://openalex.org/W2004233096","authors":[],"funders":[],"total_grants":0,"fwci":3.3934,"citation_percentile":0.91310345,"influential_citations":7,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":4},{"year":2014,"count":1},{"year":2016,"count":4},{"year":2017,"count":3},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2022,"count":4},{"year":2023,"count":1},{"year":2025,"count":2}],"oa_status":"closed","license":"http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining","oa_locations":[{"url":"http://www.nrcresearchpress.com/doi/pdf/10.1139/m85-100","host_type":"publisher"},{"url":"https://doi.org/10.1139/m85-100","host_type":"journal"}],"fields_of_study":["Biofuel production and bioconversion","Enzyme Production and Characterization","Bacteriophages and microbial interactions","Chemistry","Biology","Environmental Science"],"mesh_terms":[],"keywords":["Xylobiose","Xylanase","Ammonium sulfate precipitation","Sephadex","Size-exclusion chromatography","Chemistry","Hydrolysis","Xylan","Biochemistry","Chromatography","Enzyme","Ammonium","Xylose","Fermentation","Organic chemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-04T05:26:20.635873Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}