{"doi":"10.1139/m84-157","title":"Immunogold electron microscopy of surface antigens of oral bacteria","abstract":"<jats:p> Colloidal gold particles 3–6 nm in diameter were prepared and stabilized with the IgG fraction of polyspecific rabbit antisera produced against four different oral bacteria. The immunogold markers were used in homologous reactions to label the bacteria in a preembedding procedure for electron microscopy. An indirect immunofluorescence procedure was concurrently used to optimize the labelling conditions before observation with the electron microscope. The immunogold markers labelled fibrillar structures extending outward 50–275 nm from the Gram-positive cell envelopes and a fuzzy 5–10 nm thick capsulelike layer on the outer aspect of Bacteroides gingivalis. The immunogold method appears to be a simple, rapid, and inexpensive procedure suitable for the study of bacterial surface antigens and can be upgraded with the use of monospecific antibodies. </jats:p>","journal":"Canadian Journal of Microbiology","year":1984,"id":661599,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1727184,"name":"L. 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The immunogold method appears to be a simple, rapid, and inexpensive procedure suitable for the study of bacterial surface antigens and can be upgraded with the use of monospecific antibodies. </jats:p>","is_dataset_classified":null,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"6093974","pmcid":null,"openalex_id":"https://openalex.org/W2039630312","authors":[],"funders":[],"total_grants":0,"fwci":1.1022,"citation_percentile":0.75615873,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining","oa_locations":[{"url":"http://www.nrcresearchpress.com/doi/pdf/10.1139/m84-157","host_type":"publisher"},{"url":"https://doi.org/10.1139/m84-157","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/6093974","host_type":"repository"}],"fields_of_study":["Biosensors and Analytical Detection","Gold and Silver Nanoparticles Synthesis and Applications","Biochemical and Structural Characterization","Animals","Antigens, Bacterial","Antigens, Surface","Bacteroides","Fluorescent Antibody Technique","Gold Colloid, Radioactive","Immune Sera","Immunoglobulin G","Microscopy, Electron","Rabbits","Streptococcus","Streptococcus sanguis"],"mesh_terms":["Animals","Antigens, Bacterial","Antigens, Surface","Bacteroides","Fluorescent Antibody Technique","Gold Colloid, Radioactive","Immunoglobulin G","Immune Sera","Microscopy, Electron","Rabbits","Streptococcus","Streptococcus sanguis"],"keywords":["Immunogold labelling","Electron microscope","Antiserum","Bacteria","Bacterial cell structure","Immunofluorescence","Scanning electron microscope","Immunoelectron microscopy","Bacteroides","Antigen","Microbiology","Microscopy","Transmission electron microscopy","Biology","Segmented filamentous bacteria","Chemistry","Biophysics","Antibody","Materials science","Nanotechnology","Pathology","Immunology","Optics","Medicine","Physics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T11:17:09.426557Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}