{"doi":"10.1128/spectrum.02285-22","title":"Capturing Structural Variants of Herpes Simplex Virus Genome in Full Length by Oxford Nanopore Sequencing","abstract":"With the advent of next-generation sequencing platforms, genome sequencing of viruses can be performed in a relatively shorter time frame in even the most austere conditions. Ultralong read sequencing platforms, such as Oxford Nanopore Technology (ONT), have made it possible to capture the full-length genome of DNA viruses as a single read. By optimizing ONT for this purpose, we captured the genome (~154 Kb) of a clinical strain of herpes simplex virus 1 (HSV-1). Additionally, we captured full-length sequences of the four isomers of lab-grown HSV-1 virus and were able to determine the frequency of each within the isogenic population. This method will open new directions in studying the significance of these isomers and their clinical relevance to HSV-1 infections. It will also improve basic studies on the recombination and replication of this virus.","journal":"Microbiology Spectrum","year":2022,"id":281633,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.7751,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":957326,"name":"Emmanuel Asare","orcid":null,"position":1,"is_corresponding":false},{"id":956855,"name":"Lawrence W. Leung","orcid":"0000-0003-3333-3242","position":2,"is_corresponding":false},{"id":957327,"name":"Anna Paula de Oliveira","orcid":null,"position":3,"is_corresponding":false},{"id":957328,"name":"Katherine E. Kaugars","orcid":null,"position":4,"is_corresponding":false},{"id":905950,"name":"Claire V. Mulholland","orcid":"0000-0002-6156-1798","position":5,"is_corresponding":false},{"id":511790,"name":"Regy Lukose","orcid":null,"position":6,"is_corresponding":false},{"id":283181,"name":"Michael Berney","orcid":"0000-0002-4833-0573","position":7,"is_corresponding":false},{"id":361264,"name":"William R. Jacobs","orcid":"0000-0003-3321-3080","position":8,"is_corresponding":false},{"id":518114,"name":"Rajagopalan Saranathan","orcid":"0000-0003-2201-8028","position":0,"is_corresponding":true}],"reference_count":20,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:29:11.698554Z","pmid":"36040163","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}