{"doi":"10.1128/spectrum.01135-23","title":"Development of Negative Controls for Fc-C-Type Lectin Receptor Probes","abstract":"While Fc-CTLRs probes are useful tools to study the interaction of CTLRs with ligands, their use is limited by the lack of appropriate negative controls in assays involving fungi and potentially other pathogens. We have developed and characterized 2 negative controls for Fc-CTLRs assays: Fc-control and a Fc-Dectin-1 mutant. In this manuscript, we characterize the use of these negative controls with zymosan, a β-glucan containing particle, and 2 human pathogenic fungi, Candida albicans yeasts and Aspergillus fumigatus conidia. We show that A. fumigatus conidia nonspecifically bind Fc-CTLRs probes, demonstrating the need for appropriate negative controls in such assays.","journal":"Microbiology Spectrum","year":2023,"id":376906,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9617,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1140411,"name":"M. Kay","orcid":null,"position":1,"is_corresponding":false},{"id":1140033,"name":"Fabián Salazar","orcid":"0000-0001-5139-2292","position":2,"is_corresponding":false},{"id":1140034,"name":"Daniel P. Conn","orcid":"0000-0002-4820-4227","position":3,"is_corresponding":false},{"id":225206,"name":"David L. Williams","orcid":"0000-0002-4586-9290","position":4,"is_corresponding":false},{"id":258765,"name":"Peter C. Cook","orcid":"0000-0002-5208-4985","position":5,"is_corresponding":false},{"id":791218,"name":"Janet A. Willment","orcid":"0000-0002-7040-0857","position":6,"is_corresponding":false},{"id":397654,"name":"Gordon D. Brown","orcid":"0000-0002-0287-5383","position":7,"is_corresponding":false},{"id":1140032,"name":"Rémi Hatinguais","orcid":"0000-0001-5978-6667","position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-19T01:16:32.368674Z","pmid":"37158741","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}