{"doi":"10.1128/spectrum.00653-23","title":"Immunization with Recombinant Accessory Protein-Deficient SARS-CoV-2 Protects against Lethal Challenge and Viral Transmission","abstract":"Live-attenuated vaccines (LAVs) are able to induce robust immune responses, including both humoral and cellular immunity, representing a very promising option to provide broad and long-term immunity. To develop LAVs for SARS-CoV-2, we engineered attenuated recombinant SARS-CoV-2 (rSARS-CoV-2) that simultaneously lacks the viral open reading frame 3a (ORF3a) in combination with either ORF6, ORF7a, or ORF7b (Δ3a/Δ6, Δ3a/Δ7a, and Δ3a/Δ7b, respectively) proteins. Among them, the rSARS-CoV-2 Δ3a/Δ7b was completely attenuated and able to provide 100% protection against an otherwise lethal challenge in K18 hACE2 transgenic mice. Moreover, the rSARS-CoV-2 Δ3a/Δ7b conferred protection against viral transmission between golden Syrian hamsters.","journal":"Microbiology Spectrum","year":2023,"id":335345,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9566,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":240057,"name":"Jun‐Gyu Park","orcid":"0000-0001-7163-3591","position":1,"is_corresponding":false},{"id":277648,"name":"Kevin Chiem","orcid":"0000-0002-3892-5944","position":2,"is_corresponding":false},{"id":651167,"name":"Piyush Dravid","orcid":"0000-0002-4790-9591","position":3,"is_corresponding":false},{"id":415937,"name":"Anna Allué‐Guardia","orcid":"0000-0002-1941-2545","position":4,"is_corresponding":false},{"id":245273,"name":"Andreu Garcia‐Vilanova","orcid":"0000-0002-2385-8718","position":5,"is_corresponding":false},{"id":679532,"name":"Paula Pino Tamayo","orcid":null,"position":6,"is_corresponding":false},{"id":678868,"name":"Vinay Shivanna","orcid":"0000-0002-4625-954X","position":7,"is_corresponding":false},{"id":510473,"name":"Amit Kapoor","orcid":"0000-0002-8117-7347","position":8,"is_corresponding":false},{"id":258415,"name":"Mark R. Walter","orcid":"0000-0003-2163-5550","position":9,"is_corresponding":false},{"id":432142,"name":"James J. Kobie","orcid":"0000-0001-8069-0272","position":10,"is_corresponding":false},{"id":228962,"name":"Richard K. Plemper","orcid":"0000-0003-2034-2107","position":11,"is_corresponding":false},{"id":245288,"name":"Jordi B. Torrelles","orcid":"0000-0001-7702-5941","position":12,"is_corresponding":false},{"id":2423,"name":"Luis Martínez‐Sobrido","orcid":"0000-0001-7084-0804","position":13,"is_corresponding":false},{"id":2361,"name":"Chengjin Ye","orcid":"0000-0002-1934-9494","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":null,"created_at":"2026-07-19T01:09:58.338267Z","pmid":"37191507","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}