{"doi":"10.1128/msystems.01466-21","title":"High-Content Screening and Computational Prediction Reveal Viral Genes That Suppress the Innate Immune Response","abstract":"Infectious diseases caused by viral pathogens exacerbate health care and economic burdens. Numerous viral biomolecules suppress the human innate immune system, enabling viruses to evade an immune response from the host. Despite our current understanding of viral replications and immune evasion, new viral proteins, including those encoded by uncultivated viruses or emerging viruses, are being unearthed at a rapid pace from large-scale sequencing and surveillance projects. The use of medium- and high-throughput functional assays to characterize immunosuppressive functions of viral proteins can advance our understanding of viral replication and possibly treatment of infections. In this study, we assembled a large viral-gene library from diverse viral families and developed a high-content assay to test for inhibition of innate immunity pathways. Our work expands the tools that can rapidly link sequence and protein function, representing a practical step toward early-stage evaluation of emerging and understudied viruses.","journal":"mSystems","year":2022,"id":265710,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9507,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":833373,"name":"Erika J. Olson","orcid":null,"position":1,"is_corresponding":false},{"id":568344,"name":"Tae Yeon Yoo","orcid":"0000-0002-8145-1051","position":2,"is_corresponding":false},{"id":833374,"name":"H. Sloane Weiss","orcid":null,"position":3,"is_corresponding":false},{"id":833375,"name":"Yukiye Koide","orcid":null,"position":4,"is_corresponding":false},{"id":506393,"name":"Peter D. Koch","orcid":"0000-0001-8312-1657","position":5,"is_corresponding":false},{"id":279647,"name":"Nathan Rollins","orcid":"0000-0002-8037-6045","position":6,"is_corresponding":false},{"id":832921,"name":"Pia Mach","orcid":"0000-0003-1741-7104","position":7,"is_corresponding":false},{"id":833376,"name":"Tobias Meisinger","orcid":null,"position":8,"is_corresponding":false},{"id":300357,"name":"Trenton Bricken","orcid":null,"position":9,"is_corresponding":false},{"id":832922,"name":"Timothy Z. Chang","orcid":"0000-0002-5620-6062","position":10,"is_corresponding":false},{"id":832923,"name":"Colin Molloy","orcid":"0000-0001-7179-7721","position":11,"is_corresponding":false},{"id":833377,"name":"Jérôme Zürcher","orcid":null,"position":12,"is_corresponding":false},{"id":5437,"name":"Roger L. Chang","orcid":"0000-0003-1630-6584","position":13,"is_corresponding":false},{"id":362447,"name":"Timothy J. Mitchison","orcid":"0000-0001-7781-1897","position":14,"is_corresponding":false},{"id":674093,"name":"John I. Glass","orcid":"0000-0002-3784-5301","position":15,"is_corresponding":false},{"id":56406,"name":"Debora S. Marks","orcid":"0000-0001-9388-2281","position":16,"is_corresponding":false},{"id":315628,"name":"Jeffrey C. Way","orcid":"0000-0002-6676-3188","position":17,"is_corresponding":false},{"id":315629,"name":"Pamela A. Silver","orcid":"0000-0002-7856-4071","position":18,"is_corresponding":false},{"id":832920,"name":"Tai L. Ng","orcid":"0000-0003-1763-5590","position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":null,"created_at":"2026-07-19T00:26:49.862989Z","pmid":"35319251","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}