{"doi":"10.1128/msystems.01329-20","title":"Evaluation of the Effect of Storage Methods on Fecal, Saliva, and Skin Microbiome Composition","abstract":"Expanding our knowledge of microbial communities across diverse environments includes collecting samples in places far from the laboratory. Identifying cost-effective preservatives that will enable room temperature storage of microbial communities for sequencing analysis is crucial to enabling microbiome analyses across diverse populations. Here, we validate findings that 95% ethanol efficiently preserves microbial composition at room temperature for weeks. We also identified the optimal ratio of 95% ethanol to sample for stool and saliva to preserve both microbial load and composition. These results provide rationale for an accessible, nontoxic, cost-effective solution that will enable crowdsourcing microbiome studies, such as The Microsetta Initiative, and lower the barrier for collecting diverse samples.","journal":"mSystems","year":2021,"id":151641,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":81,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9438,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":385677,"name":"Kellen Cavagnero","orcid":"0000-0003-4160-6394","position":1,"is_corresponding":false},{"id":552309,"name":"Se Jin Song","orcid":"0000-0003-0750-5709","position":2,"is_corresponding":false},{"id":3535,"name":"Daniel McDonald","orcid":"0000-0003-0876-9060","position":3,"is_corresponding":false},{"id":347187,"name":"Stephen Wandro","orcid":"0000-0001-5514-0244","position":4,"is_corresponding":false},{"id":229202,"name":"Greg Humphrey","orcid":null,"position":5,"is_corresponding":false},{"id":555815,"name":"MacKenzie Bryant","orcid":"0000-0003-0749-2995","position":6,"is_corresponding":false},{"id":3537,"name":"Gail Ackermann","orcid":"0000-0002-3901-4931","position":7,"is_corresponding":false},{"id":558438,"name":"Edgar Diaz","orcid":null,"position":8,"is_corresponding":false},{"id":3554,"name":"Rob Knight","orcid":"0000-0002-0975-9019","position":9,"is_corresponding":false},{"id":499161,"name":"Clarisse Marotz","orcid":"0000-0002-1520-8854","position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":null,"created_at":"2026-07-18T23:43:16.823357Z","pmid":"33906915","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}