{"doi":"10.1128/msystems.01029-22","title":"Effects of Variation in Urine Sample Storage Conditions on 16S Urogenital Microbiome Analyses","abstract":"The urogenital microbiome is an understudied yet important human microbiome niche. Research has been stimulated by the relatively recent discovery that urine is not sterile; urinary tract microbes have been linked to health problems, including urinary infections, incontinence, and cancer. The quality of life and economic impact of UTIs and urgency incontinence alone are enormous, with $3.5 billion and $82.6 billion, respectively, spent in the United States. annually. Given the low biomass of urine, novelty of the field, and limited reproducibility evidence, it is critical to study urine sample storage conditions to optimize scientific rigor. Efficient and reliable preservation methods inform methods for home self-sample collection and shipping, increasing the potential use in larger-scale studies. Here, we examined both buffer and temperature variation effects on 16S rRNA gene amplicon sequencing results from urogenital samples, providing data on the consequences of common storage methods on urogenital microbiome results.","journal":"mSystems","year":2022,"id":268169,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9521,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":555815,"name":"MacKenzie Bryant","orcid":"0000-0003-0749-2995","position":1,"is_corresponding":false},{"id":555807,"name":"Kalen Cantrell","orcid":"0000-0002-6262-1668","position":2,"is_corresponding":false},{"id":552309,"name":"Se Jin Song","orcid":"0000-0003-0750-5709","position":3,"is_corresponding":false},{"id":3535,"name":"Daniel McDonald","orcid":"0000-0003-0876-9060","position":4,"is_corresponding":false},{"id":805355,"name":"Helena M. Tubb","orcid":"0000-0002-7637-5090","position":5,"is_corresponding":false},{"id":805354,"name":"Sawyer Farmer","orcid":"0009-0006-4160-9535","position":6,"is_corresponding":false},{"id":598807,"name":"Emily S. Lukacz","orcid":"0000-0002-2189-8927","position":7,"is_corresponding":false},{"id":293242,"name":"Linda Brubaker","orcid":"0000-0002-8598-2589","position":8,"is_corresponding":false},{"id":3554,"name":"Rob Knight","orcid":"0000-0002-0975-9019","position":9,"is_corresponding":false},{"id":429560,"name":"Tanya Kumar","orcid":null,"position":0,"is_corresponding":true}],"reference_count":25,"raw_metadata":null,"created_at":"2026-07-19T00:27:10.233250Z","pmid":"36475896","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}