{"doi":"10.1128/msphere.00244-20","title":"Tetanus Toxin <i>cis</i> -Loop Contributes to Light-Chain Translocation","abstract":"How protein toxins translocate their catalytic domain across a cell membrane is the least understood step in toxin action. This study utilized a reporter, β-lactamase, that was genetically fused to full-length, nontoxic tetanus toxin (βlac-TT) in discovery-based live-cell assays to study LC translocation. Directed mutagenesis identified a role for K 768 in LC translocation. K 768 was located between α15 and α16 (termed the cis -loop). Cellular assays showed that K 768 did not interfere with other toxin functions, including cell binding, intracellular trafficking, and pore formation. The equivalent K 768 is conserved among the clostridial neurotoxin family of proteins as a conserved structural motif. The cis -loop appears to contribute to LC translocation.","journal":"mSphere","year":2020,"id":86489,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9518,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":442512,"name":"Matthew S. Bluma","orcid":null,"position":1,"is_corresponding":false},{"id":441295,"name":"Joseph Barbieri","orcid":"0000-0002-9364-7465","position":2,"is_corresponding":false},{"id":442511,"name":"Madison Zuverink","orcid":null,"position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":null,"created_at":"2026-07-18T21:59:03.706190Z","pmid":"32376703","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}