{"doi":"10.1128/msphere.00069-23","title":"Identification of Efflux Substrates Using a Riboswitch-Based Reporter in Pseudomonas aeruginosa","abstract":"Treatment of Pseudomonas aeruginosa infections has become increasingly challenging. The development of novel antibiotics against this multidrug-resistant bacterium is a priority, but many drug candidates never achieve effective concentrations in the bacterial cell due to its highly restrictive outer membrane and the action of multiple efflux pumps. Here, we develop a robust and simple reporter system in P. aeruginosa to screen chemical libraries and identify compounds that either enter the cell and remain inside or enter the cell and are exported by efflux systems. This approach enables the development of rules of compound uptake and retention in P. aeruginosa that will lead to more rational design of novel antibiotics.","journal":"mSphere","year":2023,"id":383116,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9485,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1101578,"name":"Randy Hamchand","orcid":"0000-0002-9078-7550","position":1,"is_corresponding":false},{"id":1095127,"name":"Karen Anthony","orcid":"0009-0008-1848-0686","position":2,"is_corresponding":false},{"id":304470,"name":"Jason M. Crawford","orcid":"0000-0002-7583-1242","position":3,"is_corresponding":false},{"id":1095566,"name":"Alan G. Sutherland","orcid":null,"position":4,"is_corresponding":false},{"id":225255,"name":"Barbara I. Kazmierczak","orcid":"0000-0002-8162-803X","position":5,"is_corresponding":false},{"id":1150328,"name":"Verónica Urdaneta-Páez","orcid":null,"position":0,"is_corresponding":true}],"reference_count":84,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:17:25.158896Z","pmid":"36946743","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}