{"doi":"10.1128/mmbr.63.1.161-173.1999","title":"Protein Targeting to the Bacterial Cytoplasmic Membrane","abstract":"\n SUMMARY\n Proteins that perform their activity within the cytoplasmic membrane or outside this cell boundary must be targeted to the translocation site prior to their insertion and/or translocation. In bacteria, several targeting routes are known; the SecB- and the signal recognition particle-dependent pathways are the best characterized. Recently, evidence for the existence of a third major route, the twin-Arg pathway, was gathered. Proteins that use either one of these three different pathways possess special features that enable their specific interaction with the components of the targeting routes. Such targeting information is often contained in an N-terminal extension, the signal sequence, but can also be found within the mature domain of the targeted protein. Once the nascent chain starts to emerge from the ribosome, competition for the protein between different targeting factors begins. After recognition and binding, the targeting factor delivers the protein to the translocation sites at the cytoplasmic membrane. Only by means of a specific interaction between the targeting component and its receptor is the cargo released for further processing and translocation. This mechanism ensures the high-fidelity targeting of premembrane and membrane proteins to the translocation site.\n ","journal":"Microbiology and Molecular Biology Reviews","year":1999,"id":37045,"datarank":13.572052242396792,"base_score":5.594711379601839,"endowment":5.594711379601839,"self_citation_contribution":0.839206706940276,"citation_network_contribution":12.732845535456516,"self_endowment_contribution":0.839206706940276,"citer_contribution":12.732845535456516,"corpus_percentile":null,"corpus_rank":null,"citation_count":268,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":2,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":186466,"name":"Arnold J. M. Driessen","orcid":null,"position":1,"is_corresponding":false},{"id":186465,"name":"Peter Fekkes","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":5.594711379601839,"endowment":5.594711379601839,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10066835","pmcid":"PMC98961","openalex_id":"https://openalex.org/W2128366869","authors":[],"funders":[],"total_grants":0,"fwci":14.0178,"citation_percentile":0.99305677,"influential_citations":11,"citation_trend":[{"year":2012,"count":7},{"year":2013,"count":10},{"year":2014,"count":6},{"year":2015,"count":5},{"year":2016,"count":3},{"year":2017,"count":8},{"year":2018,"count":5},{"year":2019,"count":6},{"year":2020,"count":4},{"year":2021,"count":2},{"year":2022,"count":2},{"year":2024,"count":4},{"year":2025,"count":6}],"oa_status":"bronze","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://mmbr.asm.org/content/mmbr/63/1/161.full.pdf","host_type":"journal"},{"url":"https://mmbr.asm.org/content/mmbr/63/1/161.full.pdf","host_type":"BRONZE"},{"url":"https://mmbr.asm.org/content/mmbr/63/1/161.full.pdf","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/MMBR.63.1.161-173.1999","host_type":"publisher"},{"url":"https://doi.org/10.1128/mmbr.63.1.161-173.1999","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10066835","host_type":"repository"},{"url":"https://research.rug.nl/en/publications/c10a9701-5402-486e-8c81-84016b2b4b17","host_type":"repository"},{"url":"https://hdl.handle.net/11370/c10a9701-5402-486e-8c81-84016b2b4b17","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.335.583","host_type":""},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/98961","host_type":"repository"},{"url":"https://research.rug.nl/files/9905921/1999MicrobiolMolBiolRevFekkes.pdf","host_type":"repository"}],"fields_of_study":["Bacterial Genetics and Biotechnology","RNA and protein synthesis mechanisms","Toxin Mechanisms and Immunotoxins","Biology","Medicine","Chemistry","Bacterial Outer Membrane Proteins","DNA-Binding Proteins","Drosophila Proteins","Gram-Positive Bacteria","Peptidyl Transferases","Transcription Factors"],"mesh_terms":["Bacterial Outer Membrane Proteins","DNA-Binding Proteins","Gram-Positive Bacteria","Peptidyl Transferases","Transcription Factors","Drosophila Proteins"],"keywords":["Signal recognition particle","Biology","Protein targeting","Cell biology","Cytoplasm","Transport protein","Chromosomal translocation","Signal recognition particle receptor","Translocon","Protein Sorting Signals","Ribosome","Twin-arginine translocation pathway","Membrane protein","Signal peptide","Computational biology","Biochemistry","Membrane transport protein","Peptide sequence","Membrane","RNA"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.15048993.v1","title":"Additional file 1 of A cell engineering approach to enzyme-based fed-batch fermentation","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.15048993","title":"Additional file 1 of A cell engineering approach to enzyme-based fed-batch fermentation","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T18:15:30.356616Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}