{"doi":"10.1128/mcb.6.9.3042","title":"Promoter region of interleukin-2 gene undergoes chromatin structure changes and confers inducibility on chloramphenicol acetyltransferase gene during activation of T cells.","abstract":"<jats:p>The chromatin structure of the interleukin-2 (IL-2) gene was probed by DNase I treatment of isolated nuclei. The 5' region of the IL-2 gene contains three regions of hypersensitivity to DNase I. When peripheral blood T cells or Jurkat T cells are stimulated with mitogens, IL-2 message is induced, and the promoter region of the IL-2 gene develops an additional hypersensitive site. This suggests that a DNA sequence close to the transcriptional start site is involved in the transduction of the extracellular signal. Such a conclusion is further supported by DNA transfection experiments. A short segment of DNA, which includes the region of induced hypersensitivity, confers inducibility on the linked chloramphenicol acetyltransferase gene in transiently transfected Jurkat cells. In addition, cells of nonhematopoietic origins exhibit a strikingly different chromatin pattern of IL-2, suggesting a role during differentiation for some of the hypersensitive sites.</jats:p>","journal":"Molecular and Cellular Biology","year":1986,"id":20835,"datarank":10.718306903942892,"base_score":5.1298987149230735,"endowment":5.1298987149230735,"self_citation_contribution":0.7694848072384611,"citation_network_contribution":9.948822096704431,"self_endowment_contribution":0.7694848072384611,"citer_contribution":9.948822096704431,"corpus_percentile":null,"corpus_rank":null,"citation_count":168,"citer_count":146,"citers_with_citation_signal":140,"citers_with_endowment":140,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":136399,"name":"D B Durand","orcid":null,"position":1,"is_corresponding":false},{"id":136400,"name":"P Bressler","orcid":null,"position":2,"is_corresponding":false},{"id":136401,"name":"N J Holbrook","orcid":null,"position":3,"is_corresponding":false},{"id":136402,"name":"C A Norris","orcid":null,"position":4,"is_corresponding":false},{"id":136403,"name":"M Kamoun","orcid":null,"position":5,"is_corresponding":false},{"id":136404,"name":"J A Kant","orcid":null,"position":6,"is_corresponding":false},{"id":136405,"name":"G R Crabtree","orcid":null,"position":7,"is_corresponding":false},{"id":136398,"name":"U Siebenlist","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":5.1298987149230735,"endowment":5.1298987149230735,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"3491296","pmcid":null,"openalex_id":"https://openalex.org/W2138006614","authors":[],"funders":[],"total_grants":0,"fwci":4.1893,"citation_percentile":0.94947836,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2014,"count":2},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2023,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/367038","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/367038","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1128/MCB.6.9.3042","host_type":"publisher"},{"url":"https://doi.org/10.1128/mcb.6.9.3042","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/3491296","host_type":"repository"}],"fields_of_study":["Immune Cell Function and Interaction","T-cell and Retrovirus Studies","T-cell and B-cell Immunology"],"mesh_terms":["Acetyltransferases","Amino Acid Sequence","Base Sequence","Cell Line","Cells, Cultured","Chromatin","Enzyme Induction","Genes","Humans","Interleukin-2","Lymphocyte Activation","Plasmids","Promoter Regions, Genetic","T-Lymphocytes","Tetradecanoylphorbol Acetate","Chloramphenicol O-Acetyltransferase"],"keywords":["Biology","Chloramphenicol acetyltransferase","Hypersensitive site","Jurkat cells","Chromatin","Molecular biology","Transfection","DNase I hypersensitive site","Gene","Deoxyribonuclease I","DNA","Promoter","Gene expression","Cell biology","Genetics","T cell"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-06T12:15:41.216071Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}