{"doi":"10.1128/mcb.22.8.2632-2641.2002","title":"Direct Channeling of Retinoic Acid between Cellular Retinoic Acid-Binding Protein II and Retinoic Acid Receptor Sensitizes Mammary Carcinoma Cells to Retinoic Acid-Induced Growth Arrest","abstract":null,"journal":"Molecular and Cellular Biology","year":2002,"id":634544,"datarank":0.8494440720203921,"base_score":5.662960480135946,"endowment":5.662960480135946,"self_citation_contribution":0.8494440720203921,"citation_network_contribution":0.0,"self_endowment_contribution":0.8494440720203921,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":287,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1645777,"name":"Noa Noy","orcid":null,"position":1,"is_corresponding":false},{"id":1645776,"name":"Anuradha S. Budhu","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Direct Channeling of Retinoic Acid between Cellular Retinoic Acid-Binding Protein II and Retinoic Acid Receptor Sensitizes Mammary Carcinoma Cells to Retinoic Acid-Induced Growth Arrest","abstract":"Cellular retinoic acid-binding protein II (CRABP-II) is an intracellular lipid-binding protein that associates with retinoic acid with a subnanomolar affinity. We previously showed that CRABP-II enhances the transcriptional activity of the nuclear receptor with which it shares a common ligand, namely, the retinoic acid receptor (RAR), and we suggested that it may act by delivering retinoic acid to this receptor. Here, the mechanisms underlying the effects of CRABP-II on the transcriptional activity of RAR and the functional consequences of these effects were studied. We show that CRABP-II, a predominantly cytosolic protein, massively undergoes nuclear localization upon binding of retinoic acid; that it interacts with RAR in a ligand-dependent fashion; and that, in the presence of retinoic acid, the CRABP-II-RAR complex is a short-lived intermediate. The data establish that potentiation of the transcriptional activity of RAR stems directly from the ability of CRABP-II to channel retinoic acid to the receptor. We demonstrate further that overexpression of CRABP-II in MCF-7 mammary carcinoma cells dramatically enhances their sensitivity to retinoic acid-induced growth inhibition. Conversely, diminished expression of CRABP-II renders these cells retinoic acid resistant. Taken together, the data unequivocally establish the function of CRABP-II in modulating the RAR-mediated biological activities of retinoic acid.","is_dataset_classified":null,"base_score":5.662960480135946,"endowment":5.662960480135946,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11909957","pmcid":"PMC133717","openalex_id":"https://openalex.org/W2122256734","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA68150","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"5-T32-DK07158","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA068150","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"T32 DK007158","title":null}],"total_grants":4,"fwci":4.4905,"citation_percentile":0.95541634,"influential_citations":0,"citation_trend":[{"year":2012,"count":19},{"year":2013,"count":11},{"year":2014,"count":18},{"year":2015,"count":14},{"year":2016,"count":16},{"year":2017,"count":8},{"year":2018,"count":11},{"year":2019,"count":6},{"year":2020,"count":6},{"year":2021,"count":10},{"year":2022,"count":5},{"year":2023,"count":5},{"year":2024,"count":6},{"year":2025,"count":2},{"year":2026,"count":3}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/133717","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/133717","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/MCB.22.8.2632-2641.2002","host_type":"publisher"},{"url":"https://www.tandfonline.com/doi/pdf/10.1128/MCB.22.8.2632-2641.2002","host_type":"publisher"},{"url":"https://doi.org/10.1128/mcb.22.8.2632-2641.2002","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11909957","host_type":"repository"}],"fields_of_study":["Retinoids in leukemia and cellular processes","Estrogen and related hormone effects","Drug Transport and Resistance Mechanisms","Animals","Biological Transport, Active","Breast Neoplasms","COS Cells","Cell Division","Female","Humans","In Vitro Techniques","Ligands","Mice","Models, Biological","Receptors, Retinoic Acid","Recombinant Fusion Proteins","Transcription, Genetic","Tretinoin"],"mesh_terms":["Animals","Biological Transport, Active","Breast Neoplasms","Cell Division","Female","Humans","Ligands","Models, Biological","Recombinant Fusion Proteins","Transcription, Genetic","Tretinoin","Receptors, Retinoic Acid","COS Cells","Mice","In Vitro Techniques"],"keywords":["Retinoic acid","Retinoic acid receptor","Retinoic acid receptor gamma","Retinoic acid-inducible orphan G protein-coupled receptor","Retinoic acid receptor beta","Biology","Retinoid X receptor gamma","Retinoic acid receptor alpha","Nuclear receptor","Cell biology","Tretinoin","Retinoid X receptor","Biochemistry","Molecular biology","Transcription factor"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T13:48:41.994204Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}