{"doi":"10.1128/mcb.2.9.1044","title":"Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.","abstract":"<jats:p>We constructed a series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells. The prototype recombinant in this series, pSV2-cat, consisted of the beta-lactamase gene and origin of replication from pBR322 coupled to a simian virus 40 (SV40) early transcription region into which CAT coding sequences were inserted. Readily measured levels of CAT accumulated within 48 h after the introduction of pSV2-cat DNA into African green monkey kidney CV-1 cells. Because endogenous CAT activity is not present in CV-1 or other mammalian cells, and because rapid, sensitive assays for CAT activity are available, these recombinants provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells. To demonstrate the usefulness of this system, we constructed derivatives of pSV2-cat from which part or all of the SV40 promoter region was removed. Deletion of one copy of the 72-base-pair repeat sequence in the SV40 promoter caused no significant decrease in CAT synthesis in monkey kidney CV-1 cells; however, an additional deletion of 50 base pairs from the second copy of the repeats reduced CAT synthesis to 11% of its level in the wild type. We also constructed a recombinant, pSV0-cat, in which the entire SV40 promoter region was removed and a unique HindIII site was substituted for the insertion of other promoter sequences.</jats:p>","journal":"Molecular and Cellular Biology","year":1982,"id":4698,"datarank":27.067385589579477,"base_score":8.956479847999912,"endowment":8.956479847999912,"self_citation_contribution":1.343471977199987,"citation_network_contribution":25.72391361237949,"self_endowment_contribution":1.343471977199987,"citer_contribution":25.72391361237949,"corpus_percentile":96.4,"corpus_rank":2427,"citation_count":7757,"citer_count":199,"citers_with_citation_signal":199,"citers_with_endowment":199,"datacite_reuse_total":0,"is_dataset":false,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"1982-09-01","authors":[{"id":49603,"name":"L F Moffat","orcid":null,"position":1,"is_corresponding":false},{"id":49605,"name":"C Gorman","orcid":null,"position":3,"is_corresponding":false},{"id":49606,"name":"Lindsey Moffat","orcid":null,"position":4,"is_corresponding":false},{"id":49607,"name":"Bruce H. Howard","orcid":"0000-0001-9988-9724","position":5,"is_corresponding":false},{"id":49602,"name":"C M Gorman","orcid":null,"position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}