{"doi":"10.1128/mcb.16.3.839","title":"The Regulatory Domain of Human Heat Shock Factor 1 Is Sufficient To Sense Heat Stress","abstract":null,"journal":"Molecular and Cellular Biology","year":1996,"id":684915,"datarank":0.7423139835567254,"base_score":4.948759890378168,"endowment":4.948759890378168,"self_citation_contribution":0.7423139835567254,"citation_network_contribution":0.0,"self_endowment_contribution":0.7423139835567254,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":140,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1789433,"name":"Ursula Knauf","orcid":null,"position":1,"is_corresponding":false},{"id":1789434,"name":"Marie Green","orcid":null,"position":2,"is_corresponding":false},{"id":23407,"name":"Robert E. Kingston","orcid":"0000-0003-3628-4335","position":3,"is_corresponding":false},{"id":1789432,"name":"Elizabeth M. Newton","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The Regulatory Domain of Human Heat Shock Factor 1 Is Sufficient To Sense Heat Stress","abstract":"Heat shock factor (HSF) activates transcription in response to cellular stress. Human HSF1 has a central regulatory domain which can repress the activity of its activation domains at the control temperature and render them heat shock inducible. To determine whether the regulatory domain works in tandem with specific features of the HSF1 transcriptional activation domains, we first used deletion and point mutagenesis to define these activation domains. One of the activation domains can be reduced to just 20 amino acids. A GAL4 fusion protein containing the HSF 1 regulatory domain and this 20-amino-acid activation domain is repressed at the control temperature but potently activates transcription in response to heat shock. No specific amino acids in this activation domain are required for response to the regulatory domain; in particular, none of the potentially phosphorylated serine and threonine residues are required for heat induction, implying that heat-induced phosphorylation of the transcriptional activation domains is not required for induction. The regulatory domain is able to confer heat responsiveness to an otherwise completely heterologous chimeric activator that contains a portion of the VP16 activation domain, suggesting that the regulatory domain can sense heat in the absence of other portions of HSF1.","is_dataset_classified":null,"base_score":4.948759890378168,"endowment":4.948759890378168,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8622685","pmcid":"PMC231064","openalex_id":"https://openalex.org/W2146054400","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM43901","title":null}],"total_grants":1,"fwci":5.315,"citation_percentile":0.96561343,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":2},{"year":2014,"count":6},{"year":2015,"count":1},{"year":2016,"count":4},{"year":2017,"count":5},{"year":2018,"count":4},{"year":2019,"count":2},{"year":2020,"count":6},{"year":2021,"count":2},{"year":2022,"count":2},{"year":2023,"count":2},{"year":2025,"count":1}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/231064","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/231064","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/MCB.16.3.839","host_type":"publisher"},{"url":"https://www.tandfonline.com/doi/pdf/10.1128/MCB.16.3.839","host_type":"publisher"},{"url":"https://doi.org/10.1128/mcb.16.3.839","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8622685","host_type":"repository"}],"fields_of_study":["Heat shock proteins research","thermodynamics and calorimetric analyses","Physiological and biochemical adaptations","Amino Acid Sequence","Base Sequence","DNA-Binding Proteins","Heat Shock Transcription Factors","Heat Stress Disorders","Humans","Molecular Sequence Data","Phosphorylation","Point Mutation","Sequence Analysis","Transcription Factors","Transcriptional Activation"],"mesh_terms":["Heat Shock Transcription Factors","Amino Acid Sequence","Base Sequence","DNA-Binding Proteins","Humans","Molecular Sequence Data","Phosphorylation","Transcription Factors","Transcriptional Activation","Point Mutation","Sequence Analysis","Heat Stress Disorders"],"keywords":["HAMP domain","HSF1","Heat shock factor","Biology","Heat shock","Transcription factor","Cell biology","EGF-like domain","DNA-binding domain","Phosphorylation","Heat shock protein","B3 domain","Protein domain","Biochemistry","Hsp70","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T15:20:05.971513Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}