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Furthermore, glycerol gradients combined with affinity selection of biotinylated pre-mRNAs led us to show that elongated forms of U6 snRNAs enter the spliceosome and that some of these become shortened with time to a single species having the same characteristics as the major form of U6 snRNA present in mammalian nuclear extracts. We propose that this elongation-shortening process is related to the function of U6 snRNA in mammalian pre-mRNA splicing.</jats:p>","journal":"Molecular and Cellular Biology","year":1993,"id":589139,"datarank":2.4540882500195598,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"self_citation_contribution":0.5495342469194471,"citation_network_contribution":1.9045540031001127,"self_endowment_contribution":0.5495342469194471,"citer_contribution":1.9045540031001127,"corpus_percentile":null,"corpus_rank":null,"citation_count":38,"citer_count":36,"citers_with_citation_signal":34,"citers_with_endowment":34,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1507299,"name":"T Forne","orcid":null,"position":1,"is_corresponding":false},{"id":1507300,"name":"P Jeanteur","orcid":null,"position":2,"is_corresponding":false},{"id":1507301,"name":"G Cathala","orcid":null,"position":3,"is_corresponding":false},{"id":1507302,"name":"C Brunel","orcid":null,"position":4,"is_corresponding":false},{"id":1507298,"name":"J Tazi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Mammalian U6 small nuclear RNA undergoes 3' end modifications within the spliceosome.","abstract":"<jats:p>Mammalian U6 small nuclear RNA (snRNA) is heterogeneous with respect to the number of 3' terminal U residues. The major form terminates with five U residues and a 2',3' cyclic phosphate. Because of the presence in HeLa cell nuclear extracts of a terminal uridylyl transferase, a minor form of U6 snRNA is elongated, producing multiple species containing up to 12 U residues. In this study we have used glycerol gradients to demonstrate that these U6 snRNA forms are assembled into U6 ribonucleoprotein (RNP), U4/U6 snRNPs, and U4/U5/U6 tri-snRNP complexes. Furthermore, glycerol gradients combined with affinity selection of biotinylated pre-mRNAs led us to show that elongated forms of U6 snRNAs enter the spliceosome and that some of these become shortened with time to a single species having the same characteristics as the major form of U6 snRNA present in mammalian nuclear extracts. We propose that this elongation-shortening process is related to the function of U6 snRNA in mammalian pre-mRNA splicing.</jats:p>","is_dataset_classified":null,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8441402","pmcid":null,"openalex_id":"https://openalex.org/W2154679838","authors":[],"funders":[],"total_grants":0,"fwci":0.9451,"citation_percentile":0.74265462,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":1},{"year":2014,"count":1},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2018,"count":2},{"year":2019,"count":1},{"year":2020,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/359476","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/359476","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1128/MCB.13.3.1641","host_type":"publisher"},{"url":"https://doi.org/10.1128/mcb.13.3.1641","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8441402","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC359476","host_type":"repository"}],"fields_of_study":["RNA Research and Splicing","Biotin and Related Studies","RNA and protein synthesis mechanisms"],"mesh_terms":["Base Composition","Cell Nucleus","Cell-Free System","HeLa Cells","UDPglucose-Hexose-1-Phosphate Uridylyltransferase","Humans","Nucleic Acid Conformation","RNA Precursors","RNA Splicing","RNA, Small Nuclear","Transcription, Genetic","Uridine Monophosphate","Genetic Variation","Spliceosomes","Ribonucleoprotein, U4-U6 Small Nuclear","Macromolecular Substances"],"keywords":["snRNP","Small nuclear RNA","Prp24","Spliceosome","Biology","RNA splicing","Small nuclear ribonucleoprotein","RNA","Cell biology","Ribonucleoprotein","Precursor mRNA","Biochemistry","Biophysics","Molecular biology","RNA-dependent RNA polymerase","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-23T13:43:50.751010Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}