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The defect in poly(A)- mRNAs affected a late step in translational initiation, was distinct from the phenotype associated with cap-deficient mRNAs, and resulted in a reduced ability to form 80S initiation complexes. Moreover, poly(A) added in trans inhibited translation from capped polyadenylated mRNAs but stimulated translation from capped poly(A)- mRNAs. 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and protein synthesis mechanisms","RNA Research and Splicing","RNA modifications and cancer"],"mesh_terms":["Animals","Base Sequence","Binding, Competitive","Cell-Free System","Genetic Vectors","Globins","Kinetics","Molecular Sequence Data","Peptide Chain Elongation, Translational","Peptide Chain Initiation, Translational","Plasmids","Poly A","Rabbits","Reticulocytes","RNA Caps","RNA, Messenger","Templates, Genetic","Transcription, Genetic","Protein Biosynthesis"],"keywords":["Polysome","Polyadenylation","Biology","Messenger RNA","Translational efficiency","Translation (biology)","EIF4E","Ribonucleoprotein","Molecular biology","Poly(A)-binding protein","Initiation factor","Protein biosynthesis","Translational regulation","Ribosome","P-bodies","Eukaryotic translation","Messenger RNP","Cell 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