{"doi":"10.1128/mcb.00698-13","title":"Structure and Dimerization Properties of the Aryl Hydrocarbon Receptor PAS-A Domain","abstract":null,"journal":"Molecular and Cellular Biology","year":2013,"id":594467,"datarank":0.6892679775201885,"base_score":4.59511985013459,"endowment":4.59511985013459,"self_citation_contribution":0.6892679775201885,"citation_network_contribution":0.0,"self_endowment_contribution":0.6892679775201885,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":98,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":316028,"name":"Nalini Potluri","orcid":null,"position":1,"is_corresponding":false},{"id":231322,"name":"Youngchang Kim","orcid":"0000-0002-1610-4889","position":2,"is_corresponding":false},{"id":110633,"name":"Fraydoon Rastinejad","orcid":null,"position":3,"is_corresponding":false},{"id":89755,"name":"Dalei Wu","orcid":"0000-0003-1817-7229","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Structure and Dimerization Properties of the Aryl Hydrocarbon Receptor PAS-A Domain","abstract":"The aryl hydrocarbon receptor (AHR) is a ligand-dependent transcription factor that binds to xenobiotics and responds by regulating the expression of gene programs required for detoxification and metabolism. AHR and its heterodimerization partner aryl hydrocarbon receptor nuclear translocator (ARNT) belong to the basic helix-loop-helix (bHLH)-PER-ARNT-SIM (PAS) family of transcription factors. Here we report the 2.55-Å-resolution crystal structure of the mouse AHR PAS-A domain, which represents the first AHR-derived protein structure. The AHR PAS-A domain forms a helix-swapped homodimer in the crystal and also in solution. Through a detailed mutational analysis of all interface residues, we identified several hydrophobic residues that are important for AHR dimerization and function. Our crystallographic visualization of AHR PAS-A dimerization leads us to propose a mode of heterodimerization with ARNT that is supported by both biochemical and cell-based data. Our studies also highlight the residues of other mammalian bHLH-PAS proteins that are likely involved in their homo- or heterodimerization.","is_dataset_classified":null,"base_score":4.59511985013459,"endowment":4.59511985013459,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24001774","pmcid":"PMC3811902","openalex_id":"https://openalex.org/W2151481630","authors":[],"funders":[],"total_grants":0,"fwci":4.6769,"citation_percentile":0.9477337,"influential_citations":0,"citation_trend":[{"year":2014,"count":6},{"year":2015,"count":6},{"year":2016,"count":9},{"year":2017,"count":11},{"year":2018,"count":3},{"year":2019,"count":4},{"year":2020,"count":6},{"year":2021,"count":16},{"year":2022,"count":5},{"year":2023,"count":10},{"year":2024,"count":8},{"year":2025,"count":7},{"year":2026,"count":7}],"oa_status":"bronze","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://mcb.asm.org/content/mcb/33/21/4346.full.pdf","host_type":"journal"},{"url":"https://mcb.asm.org/content/mcb/33/21/4346.full.pdf","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/MCB.00698-13","host_type":"publisher"},{"url":"https://www.tandfonline.com/doi/pdf/10.1128/MCB.00698-13","host_type":"publisher"},{"url":"https://doi.org/10.1128/mcb.00698-13","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24001774","host_type":"repository"},{"url":"http://europepmc.org/articles/PMC3811902","host_type":"repository"},{"url":"http://mcb.asm.org/cgi/pmidlookup?view=long&pmid=24001774","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3811902","host_type":"repository"}],"fields_of_study":["Toxic Organic Pollutants Impact","Adipose Tissue and Metabolism","Effects and risks of endocrine disrupting chemicals","Amino Acid Sequence","Amino Acid Substitution","Animals","Basic Helix-Loop-Helix Proteins","Conserved Sequence","Crystallography, X-Ray","Hydrophobic and Hydrophilic Interactions","Mice","Models, Molecular","Molecular Sequence Data","Point Mutation","Protein Interaction Domains and Motifs","Protein Multimerization","Protein Structure, Quaternary","Protein Structure, Secondary","Receptors, Aryl Hydrocarbon","Sequence Homology, Amino Acid"],"mesh_terms":["Amino Acid Sequence","Animals","Models, Molecular","Molecular Sequence Data","Conserved Sequence","Point Mutation","Sequence Homology, Amino Acid","Protein Structure, Secondary","Receptors, Aryl Hydrocarbon","Crystallography, X-Ray","Amino Acid Substitution","Protein Structure, Quaternary","Mice","Basic Helix-Loop-Helix Proteins","Basic Helix-Loop-Helix Transcription Factors","Protein Interaction Domains and Motifs","Protein Multimerization","Hydrophobic and Hydrophilic Interactions"],"keywords":["Aryl hydrocarbon receptor nuclear translocator","Aryl hydrocarbon receptor","Transcription factor","Basic helix-loop-helix","Biology","PAS domain","Cell biology","Transcription (linguistics)","Protein structure","Receptor","Biochemistry","DNA-binding protein","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"uniprot"},{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T14:39:39.526418Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}