{"doi":"10.1128/mcb.00068-15","title":"Bimodal Interaction of Mammalian Polo-Like Kinase 1 and a Centrosomal Scaffold, Cep192, in the Regulation of Bipolar Spindle Formation","abstract":null,"journal":"Molecular and Cellular Biology","year":2015,"id":645433,"datarank":0.6064576901751826,"base_score":4.04305126783455,"endowment":4.04305126783455,"self_citation_contribution":0.6064576901751826,"citation_network_contribution":0.0,"self_endowment_contribution":0.6064576901751826,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":56,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":205175,"name":"Jung-Eun Park","orcid":null,"position":1,"is_corresponding":false},{"id":205176,"name":"Tae-Sung Kim","orcid":null,"position":2,"is_corresponding":false},{"id":1408058,"name":"Eun Hye Lee","orcid":"0000-0002-4206-6214","position":3,"is_corresponding":false},{"id":1680638,"name":"Suk-Youl Park","orcid":null,"position":4,"is_corresponding":false},{"id":261671,"name":"Ming Zhou","orcid":"0000-0002-7460-550X","position":5,"is_corresponding":false},{"id":205177,"name":"Jeong K. Bang","orcid":null,"position":6,"is_corresponding":false},{"id":367481,"name":"Kyung S. Lee","orcid":"0000-0001-8326-7162","position":7,"is_corresponding":false},{"id":467517,"name":"Lingjun Meng","orcid":"0000-0001-8610-5679","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Bimodal Interaction of Mammalian Polo-Like Kinase 1 and a Centrosomal Scaffold, Cep192, in the Regulation of Bipolar Spindle Formation","abstract":"Serving as microtubule-organizing centers, centrosomes play a key role in forming bipolar spindles. The mechanism of how centrosomes promote bipolar spindle assembly in various organisms remains largely unknown. A recent study with Xenopus laevis egg extracts suggested that the Plk1 ortholog Plx1 interacts with the phospho-T46 (p-T46) motif of Xenopus Cep192 (xCep192) to form an xCep192-mediated xAurA-Plx1 cascade that is critical for bipolar spindle formation. Here, we demonstrated that in cultured human cells, Cep192 recruits AurA and Plk1 in a cooperative manner, and this event is important for the reciprocal activation of AurA and Plk1. Strikingly, Plk1 interacted with Cep192 through either the p-T44 (analogous to Xenopus p-T46) or the newly identified p-S995 motif via its C-terminal noncatalytic polo-box domain. The interaction between Plk1 and the p-T44 motif was prevalent in the presence of Cep192-bound AurA, whereas the interaction of Plk1 with the p-T995 motif was preferred in the absence of AurA binding. Notably, the loss of p-T44- and p-S995-dependent Cep192-Plk1 interactions induced an additive defect in recruiting Plk1 and γ-tubulin to centrosomes, which ultimately led to a failure in proper bipolar spindle formation and mitotic progression. Thus, we propose that Plk1 promotes centrosome-based bipolar spindle formation by forming two functionally nonredundant complexes with Cep192.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26012549","pmcid":"PMC4524112","openalex_id":null,"authors":[],"funders":[{"funder_name":"Intramural NIH HHS","grant_id":"","title":null}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://mcb.asm.org/content/mcb/35/15/2626.full.pdf","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/MCB.00068-15","host_type":"publisher"},{"url":"https://www.tandfonline.com/doi/pdf/10.1128/MCB.00068-15","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["Cell Line, Tumor","Hela Cells","Centrosome","Microtubules","Humans","Tubulin","Cell Cycle Proteins","Proto-Oncogene Proteins","Chromosomal Proteins, Non-Histone","RNA, Small Interfering","RNA Interference","Binding Sites","Enzyme Activation","Amino Acid Motifs","Protein Binding","HEK293 Cells","Spindle Apparatus","Aurora Kinase A","Protein Serine-Threonine Kinases","Polo-Like Kinase 1"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-09T05:33:18.366045Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}