{"doi":"10.1128/mbio.03499-22","title":"Identification and Structural Modeling of the RNA Polymerase Omega Subunits in Chlamydiae and Other Obligate Intracellular Bacteria","abstract":"Chlamydiae are obligate intracellular bacteria that replicate only inside eukaryotic cells. Previously, it has not been possible to identify a candidate gene encoding the chlamydial RNA polymerase ω subunit, and it has been hypothesized that the chlamydial RNA polymerase ω subunit was lost in the evolutionary process through which Chlamydiae reduced their genome size and proteome sizes to adapt to an obligate intracellular lifestyle. Here, we report the identification of the chlamydial RNA polymerase ω subunit, based on conserved sequence, conserved synteny, AlphaFold-predicted conserved three-dimensional structure, and AlfaFold-Multimer-predicted conserved interactions. Our identification of the previously elusive chlamydial RNA polymerase ω subunit sets the stage for investigation of its roles in regulation of gene expression during chlamydial growth, development, and stress responses, and sets the stage for preparation and study of the intact chlamydial RNA polymerase and its interactions with inhibitors.","journal":"mBio","year":2023,"id":376459,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9459,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1007725,"name":"Danny Wan","orcid":"0000-0001-8262-8173","position":1,"is_corresponding":false},{"id":1008138,"name":"Arkaprabha Ghatak","orcid":null,"position":2,"is_corresponding":false},{"id":554381,"name":"Chengyuan Wang","orcid":"0000-0002-8605-0716","position":3,"is_corresponding":false},{"id":1008139,"name":"Deyu Feng","orcid":null,"position":4,"is_corresponding":false},{"id":914345,"name":"Joseph D. Fondell","orcid":"0000-0002-7046-9854","position":5,"is_corresponding":false},{"id":506449,"name":"Richard H. Ebright","orcid":"0000-0001-8915-7140","position":6,"is_corresponding":false},{"id":529972,"name":"Huizhou Fan","orcid":"0000-0002-4903-926X","position":7,"is_corresponding":false},{"id":852987,"name":"Andrew Y. S. Cheng","orcid":"0000-0002-9155-6156","position":0,"is_corresponding":true}],"reference_count":126,"raw_metadata":null,"created_at":"2026-07-19T01:16:28.318749Z","pmid":"36719197","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}