{"doi":"10.1128/mbio.03273-21","title":"A J Domain Protein Functions as a Histone Chaperone to Maintain Genome Integrity and the Response to DNA Damage in a Human Fungal Pathogen","abstract":"DNA replication, gene expression, and genomic repair all require precise coordination of the many proteins that interact with DNA. This includes the histones as well as their chaperones. In this study, we show that a histone chaperone, Dnj4, is required for genome integrity and for the response to DNA damage. The gene encoding this protein in Cryptococcus neoformans lacks an ortholog in Saccharomyces cerevisiae; however, it is conserved in humans in which its ortholog is essential. Since it is not essential in C. neoformans, we were able to generate deletion mutants to characterize the roles of Dnj4. We also expressed Dnj4 in S. cerevisiae, in which it was able to bind S. cerevisiae histones and interfere with existing histone chaperoning machinery. Therefore, we show a conserved role for Dnj4 in histone chaperoning that suggests that C. neoformans is useful to better understand aspects of this important biological process.","journal":"mBio","year":2021,"id":203134,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9533,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":783827,"name":"Christopher W. J. Lee","orcid":"0000-0002-1523-7882","position":1,"is_corresponding":false},{"id":783828,"name":"Kerstin Schmitt","orcid":"0000-0001-9627-031X","position":2,"is_corresponding":false},{"id":783829,"name":"Oliver Valerius","orcid":"0000-0003-4430-819X","position":3,"is_corresponding":false},{"id":783830,"name":"Guanggan Hu","orcid":"0000-0002-2274-0312","position":4,"is_corresponding":false},{"id":783831,"name":"Mélissa Caza","orcid":"0000-0002-5403-8034","position":5,"is_corresponding":false},{"id":368611,"name":"Gerhard H. Braus","orcid":"0000-0002-3117-5626","position":6,"is_corresponding":false},{"id":783832,"name":"James W. Kronstad","orcid":"0000-0003-4240-6976","position":7,"is_corresponding":false},{"id":783826,"name":"Linda C. Horianopoulos","orcid":"0000-0001-5627-2783","position":0,"is_corresponding":true}],"reference_count":68,"raw_metadata":null,"created_at":"2026-07-18T23:51:14.206397Z","pmid":"34933457","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}