{"doi":"10.1128/mbio.03230-19","title":"Pyocin S5 Import into Pseudomonas aeruginosa Reveals a Generic Mode of Bacteriocin Transport","abstract":"Bacteriocins are toxic polypeptides made by bacteria to kill their competitors, making them interesting as potential antibiotics. Here, we reveal unsuspected commonalities in bacteriocin uptake pathways, through molecular and cellular dissection of the import pathway for the pore-forming bacteriocin pyocin S5 (PyoS5), which targets Pseudomonas aeruginosa . In addition to its C-terminal pore-forming domain, PyoS5 is composed of two tandemly repeated helical domains that we also identify in other pyocins. Functional analyses demonstrate that they have distinct roles in the import process. One recognizes conserved sugars projected from the surface, while the other recognizes a specific outer membrane siderophore transporter, FptA, in the case of PyoS5. Through engineering of Escherichia coli cells, we show that pyocins can be readily repurposed to kill other species. This suggests basic ground rules for the outer membrane translocation step that likely apply to many bacteriocins targeting Gram-negative bacteria.","journal":"mBio","year":2020,"id":116503,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9552,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":439625,"name":"E.D. Lowe","orcid":"0000-0002-1757-0208","position":1,"is_corresponding":false},{"id":544230,"name":"Joseph Gault","orcid":"0000-0001-7686-2983","position":2,"is_corresponding":false},{"id":544231,"name":"Nicholas G. Housden","orcid":"0000-0002-4706-8278","position":3,"is_corresponding":false},{"id":544232,"name":"Renata Kaminska","orcid":"0000-0001-7252-379X","position":4,"is_corresponding":false},{"id":544233,"name":"Timm Weber","orcid":"0000-0003-2079-842X","position":5,"is_corresponding":false},{"id":544234,"name":"Catriona M. A. Thompson","orcid":"0000-0002-0069-2727","position":6,"is_corresponding":false},{"id":544235,"name":"Gaëtan L. A. Mislin","orcid":"0000-0002-5646-3392","position":7,"is_corresponding":false},{"id":544236,"name":"Isabelle J. Schalk","orcid":"0000-0002-8351-1679","position":8,"is_corresponding":false},{"id":544237,"name":"Daniel Walker","orcid":"0000-0002-4206-2942","position":9,"is_corresponding":false},{"id":250509,"name":"Carol V. Robinson","orcid":"0000-0001-7829-5505","position":10,"is_corresponding":false},{"id":544238,"name":"Colin Kleanthous","orcid":"0000-0002-3273-0302","position":11,"is_corresponding":false},{"id":544229,"name":"Hannah Michaela Behrens","orcid":"0000-0002-7406-7086","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-18T23:13:44.286758Z","pmid":"32156826","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}