{"doi":"10.1128/mbio.03203-19","title":"Hijacking the Fusion Complex of Human Parainfluenza Virus as an Antiviral Strategy","abstract":"Paramyxoviruses, including human parainfluenza virus type 3, are internalized into host cells by fusion between viral and target cell membranes. The receptor binding protein, hemagglutinin-neuraminidase (HN), upon binding to its cell receptor, triggers conformational changes in the fusion protein (F). This action of HN activates F to reach its fusion-competent state. Using small molecules that interact with HN, we can induce the premature activation of F and inactivate the virus. To obtain highly active pretriggering compounds, we carried out a virtual modeling screen for molecules that interact with a sialic acid binding site on HN that we propose to be the site involved in activating F. We use cryo-electron tomography of authentic intact viral particles for the first time to directly assess the mechanism of action of this treatment on the conformation of the viral F protein and present the first direct observation of the induced conformational rearrangement in the viral F protein.","journal":"mBio","year":2020,"id":75639,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":23,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9515,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":395044,"name":"Elon Yariv","orcid":"0000-0001-7457-2608","position":1,"is_corresponding":false},{"id":396302,"name":"Ksenia Rybkina","orcid":null,"position":2,"is_corresponding":false},{"id":395045,"name":"Vicente Más","orcid":"0000-0002-0887-0743","position":3,"is_corresponding":false},{"id":225930,"name":"Francesca T. Bovier","orcid":"0000-0003-3446-9619","position":4,"is_corresponding":false},{"id":275518,"name":"Amédée des Georges","orcid":"0000-0002-9704-3781","position":5,"is_corresponding":false},{"id":108342,"name":"Alexander L. Greninger","orcid":"0000-0002-7443-0527","position":6,"is_corresponding":false},{"id":395046,"name":"Christopher A. Alabi","orcid":"0000-0003-2654-018X","position":7,"is_corresponding":false},{"id":225940,"name":"Matteo Porotto","orcid":"0000-0003-3866-9220","position":8,"is_corresponding":false},{"id":395047,"name":"Nir Ben‐Tal","orcid":"0000-0001-6901-832X","position":9,"is_corresponding":false},{"id":225939,"name":"Anne Moscona","orcid":"0000-0002-1796-8320","position":10,"is_corresponding":false},{"id":395043,"name":"Tara C. Marcink","orcid":"0000-0003-4295-1229","position":0,"is_corresponding":true}],"reference_count":88,"raw_metadata":null,"created_at":"2026-07-18T21:46:55.856612Z","pmid":"32047132","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}