{"doi":"10.1128/mbio.03189-19","title":"Novel Cassette Assay To Quantify the Outer Membrane Permeability of Five β-Lactams Simultaneously in Carbapenem-Resistant<i>Klebsiella pneumoniae</i>and<i>Enterobacter cloacae</i>","abstract":"Antimicrobial resistance is causing a global human health crisis and is affecting all antibiotic classes. While β-lactams have been commonly used against susceptible isolates of Klebsiella pneumoniae and Enterobacter cloacae , carbapenem-resistant isolates are spreading worldwide and pose substantial clinical challenges. Rapid penetration of β-lactams leads to high drug concentrations at their periplasmic target sites, allowing β-lactams to more completely inactivate their target receptors. Despite this, there are limited tangible data on the permeability of β-lactams through the outer membranes of many Gram-negative pathogens. This study presents a novel, cassette assay, which can simultaneously characterize the permeability of five β-lactams in multidrug-resistant clinical isolates. We show that carbapenems, and especially imipenem, penetrate the outer membrane of K. pneumoniae and E. cloacae substantially faster than noncarbapenem β-lactams. The ability to efficiently characterize the outer membrane permeability is critical to optimize the use of β-lactams and combat carbapenem-resistant isolates.","journal":"mBio","year":2020,"id":68180,"datarank":0.47032413238937254,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.0,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9606,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":298092,"name":"Xun Tao","orcid":"0009-0004-5477-3786","position":1,"is_corresponding":false},{"id":361325,"name":"Bartolomé Moyá","orcid":"0000-0001-6404-246X","position":2,"is_corresponding":false},{"id":361326,"name":"Yuanyuan Jiao","orcid":"0009-0000-7192-2676","position":3,"is_corresponding":false},{"id":361327,"name":"Kari B. Basso","orcid":"0000-0003-1268-6655","position":4,"is_corresponding":false},{"id":300099,"name":"Jieqiang Zhou","orcid":null,"position":5,"is_corresponding":false},{"id":361328,"name":"Yinzhi Lang","orcid":"0000-0002-6857-4516","position":6,"is_corresponding":false},{"id":361329,"name":"Dhruvitkumar S. Sutaria","orcid":"0000-0001-8149-3481","position":7,"is_corresponding":false},{"id":361330,"name":"Alexandre Prehn Zavascki","orcid":"0000-0001-5331-4837","position":8,"is_corresponding":false},{"id":361331,"name":"Afonso Luís Barth","orcid":"0000-0002-7969-3908","position":9,"is_corresponding":false},{"id":361332,"name":"Stephanie M. Reeve","orcid":"0000-0003-2064-405X","position":10,"is_corresponding":false},{"id":361333,"name":"Herbert P. Schweizer","orcid":"0000-0002-8847-335X","position":11,"is_corresponding":false},{"id":361334,"name":"Deanna Deveson Lucas","orcid":"0000-0002-6457-3823","position":12,"is_corresponding":false},{"id":361335,"name":"John D. Boyce","orcid":"0000-0002-8614-3074","position":13,"is_corresponding":false},{"id":232761,"name":"Robert A. Bonomo","orcid":"0000-0002-3299-894X","position":14,"is_corresponding":false},{"id":360604,"name":"Richard Lee","orcid":"0000-0002-2397-0443","position":15,"is_corresponding":false},{"id":361336,"name":"Beom Soo Shin","orcid":"0000-0002-6706-5445","position":16,"is_corresponding":false},{"id":361337,"name":"Arnold Louie","orcid":"0000-0001-6227-7580","position":17,"is_corresponding":false},{"id":361338,"name":"George L. Drusano","orcid":"0000-0001-9162-7332","position":18,"is_corresponding":false},{"id":298093,"name":"Jürgen B. Bulitta","orcid":"0000-0001-7352-3097","position":19,"is_corresponding":false},{"id":362815,"name":"Tae Hwan Kim","orcid":null,"position":0,"is_corresponding":true}],"reference_count":63,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T21:40:52.680475Z","pmid":"32047131","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}