{"doi":"10.1128/mbio.02944-22","title":"Unraveling the Pathobiological Role of the Fungal KEOPS Complex in Cryptococcus neoformans","abstract":"The cellular function and structural configuration of the KEOPS complex have been elucidated in some eukaryotes and archaea but have never been fully characterized in fungal pathogens. Here, we comprehensively analyzed the pathobiological roles of the KEOPS complex in the globally prevalent fungal meningitis-causing pathogen C. neoformans. The CnKEOPS complex, composed of a linear arrangement of Pcc1-Kae1-Bud32-Cgi121, not only played evolutionarily conserved roles in growth, sexual development, stress responses, and tRNA modification but also had unique roles in controlling virulence factor production and pathogenicity. Notably, a unique extended loop structure in CnBud32 is critical for the KEOPS complex in C. neoformans. Supporting its pleiotropic roles, transcriptome analysis revealed that the CnKEOPS complex governs several hundreds of genes involved in carbon and amino acid metabolism, pheromone response, and ergosterol biosynthesis. Therefore, this study provides novel insights into the fungal KEOPS complex that could be exploited as a potential antifungal drug target.","journal":"mBio","year":2022,"id":276000,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9475,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":322504,"name":"Eunji Jeong","orcid":"0000-0002-9873-7726","position":1,"is_corresponding":false},{"id":322503,"name":"Dong‐Gi Lee","orcid":"0000-0002-6767-9543","position":2,"is_corresponding":false},{"id":322497,"name":"Jae‐Hyung Jin","orcid":"0000-0002-0589-9444","position":3,"is_corresponding":false},{"id":946083,"name":"Yee‐Seul So","orcid":"0000-0002-9922-3575","position":4,"is_corresponding":false},{"id":322509,"name":"Seong-Ryong Yu","orcid":"0000-0003-0025-3860","position":5,"is_corresponding":false},{"id":705820,"name":"Kyung‐Jo Lee","orcid":null,"position":6,"is_corresponding":false},{"id":806865,"name":"Hye‐Jeong Ha","orcid":"0000-0002-6506-9338","position":7,"is_corresponding":false},{"id":946084,"name":"Chi-Jan Lin","orcid":"0000-0002-6873-6570","position":8,"is_corresponding":false},{"id":53025,"name":"Ying‐Lien Chen","orcid":"0000-0002-1966-470X","position":9,"is_corresponding":false},{"id":946085,"name":"Jun Bae Park","orcid":"0000-0002-3023-1040","position":10,"is_corresponding":false},{"id":424860,"name":"Hyun‐Soo Cho","orcid":"0000-0003-4067-4715","position":11,"is_corresponding":false},{"id":304364,"name":"Anna Floyd Averette","orcid":"0000-0002-5877-0812","position":12,"is_corresponding":false},{"id":53024,"name":"Joseph Heitman","orcid":"0000-0001-6369-5995","position":13,"is_corresponding":false},{"id":946086,"name":"Kyu‐Ho Lee","orcid":"0000-0002-4424-8062","position":14,"is_corresponding":false},{"id":806869,"name":"Kangseok Lee","orcid":"0000-0002-0060-6884","position":15,"is_corresponding":false},{"id":322518,"name":"Yong‐Sun Bahn","orcid":"0000-0001-9573-5752","position":16,"is_corresponding":false},{"id":322507,"name":"Yeseul Choi","orcid":"0000-0002-9369-902X","position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":null,"created_at":"2026-07-19T00:28:26.545826Z","pmid":"36377896","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}