{"doi":"10.1128/mbio.02273-18","title":"A Genome-Wide Analysis of Adhesion in\n                    <i>Caulobacter crescentus</i>\n                    Identifies New Regulatory and Biosynthetic Components for Holdfast Assembly","abstract":"<jats:p>\n                    Bacteria routinely encounter biotic and abiotic materials in their surrounding environments, and they often enlist specific behavioral programs to colonize these materials. Adhesion is an early step in colonizing a surface.\n                    <jats:named-content content-type=\"genus-species\">Caulobacter crescentus</jats:named-content>\n                    produces a structure called the holdfast which allows this organism to attach to and colonize surfaces. To understand how the holdfast is produced, we performed a genome-wide search for genes that contribute to adhesion by selecting for mutants that could not attach to cheesecloth. We discovered complex interactions between genes that mediate surface contact and genes that contribute to holdfast development. Our genetic selection identified what likely represents a comprehensive set of genes required to generate a holdfast, laying the groundwork for a detailed characterization of the enzymes that build this specialized adhesin.\n                  </jats:p>","journal":"mBio","year":2019,"id":685803,"datarank":0.5955437870328184,"base_score":3.970291913552122,"endowment":3.970291913552122,"self_citation_contribution":0.5955437870328184,"citation_network_contribution":0.0,"self_endowment_contribution":0.5955437870328184,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":52,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":365978,"name":"Aretha Fiebig","orcid":"0000-0002-0612-5029","position":1,"is_corresponding":false},{"id":365979,"name":"Sean Crosson","orcid":"0000-0002-1727-322X","position":2,"is_corresponding":false},{"id":570984,"name":"David M. Hershey","orcid":"0000-0001-8745-9524","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A Genome-Wide Analysis of Adhesion in\n                    <i>Caulobacter crescentus</i>\n                    Identifies New Regulatory and Biosynthetic Components for Holdfast Assembly","abstract":"<jats:p>\n                    Bacteria routinely encounter biotic and abiotic materials in their surrounding environments, and they often enlist specific behavioral programs to colonize these materials. Adhesion is an early step in colonizing a surface.\n                    <jats:named-content content-type=\"genus-species\">Caulobacter crescentus</jats:named-content>\n                    produces a structure called the holdfast which allows this organism to attach to and colonize surfaces. To understand how the holdfast is produced, we performed a genome-wide search for genes that contribute to adhesion by selecting for mutants that could not attach to cheesecloth. We discovered complex interactions between genes that mediate surface contact and genes that contribute to holdfast development. Our genetic selection identified what likely represents a comprehensive set of genes required to generate a holdfast, laying the groundwork for a detailed characterization of the enzymes that build this specialized adhesin.\n                  </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30755507","pmcid":"PMC6372794","openalex_id":null,"authors":[],"funders":[{"funder_name":"HHS | National Institutes of Health","grant_id":"R01GM087353","title":null},{"funder_name":"Helen Hay Whitney Foundation","grant_id":"","title":null}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://mbio.asm.org/content/mbio/10/1/e02273-18.full.pdf","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/mBio.02273-18","host_type":"publisher"},{"url":"http://europepmc.org/pmc/articles/PMC6372794","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6372794","host_type":"repository"},{"url":"https://doaj.org/article/7edf2bec03c34ccbad0940044988bd25","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC6372794","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC6372794?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":[],"mesh_terms":["Caulobacter crescentus","Polysaccharides, Bacterial","DNA Transposable Elements","Mutagenesis, Insertional","Bacterial Adhesion","Gene Expression Regulation, Bacterial","Gene Library","Molecular Sequence Annotation"],"keywords":["Polysaccharide","adhesion","holdfast","Caulobacter","Pilus","Barseq"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"geo"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T17:42:10.198543Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}