{"doi":"10.1128/mbio.01625-20","title":"(p)ppGpp and c-di-AMP Homeostasis Is Controlled by CbpB in Listeria monocytogenes","abstract":"Bacteria must efficiently maintain homeostasis of essential molecules to survive in the environment. We found that the levels of c-di-AMP and (p)ppGpp, two nucleotide second messengers that are highly conserved throughout the microbial world, coexist in a homeostatic loop in the facultative intracellular pathogen Listeria monocytogenes . Here, we found that cyclic di-AMP binding protein B (CbpB) acts as a c-di-AMP sensor that promotes the synthesis of (p)ppGpp by binding to RelA when c-di-AMP levels are low. Addition of c-di-AMP prevented RelA activation by binding and sequestering CbpB. Previous studies showed that (p)ppGpp binds and inhibits c-di-AMP phosphodiesterases, resulting in an increase in c-di-AMP. This pathway is controlled via direct enzymatic regulation and indicates an additional mechanism of ribosome-independent stringent activation.","journal":"mBio","year":2020,"id":66275,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":52,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9509,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":352290,"name":"Megan K. M. Young","orcid":null,"position":1,"is_corresponding":false},{"id":351111,"name":"Shukun Luo","orcid":"0000-0002-2766-3125","position":2,"is_corresponding":false},{"id":351112,"name":"Jeffrey Wang","orcid":"0000-0003-0501-8683","position":3,"is_corresponding":false},{"id":239473,"name":"Aaron T. Whiteley","orcid":"0000-0002-0075-7519","position":4,"is_corresponding":false},{"id":331579,"name":"Joshua J. Woodward","orcid":"0000-0002-4630-403X","position":5,"is_corresponding":false},{"id":86983,"name":"Liang Tong","orcid":"0000-0002-0563-6468","position":6,"is_corresponding":false},{"id":351113,"name":"Jue D. Wang","orcid":"0000-0003-1503-170X","position":7,"is_corresponding":false},{"id":351114,"name":"Daniel A. Portnoy","orcid":"0000-0003-1218-2799","position":8,"is_corresponding":false},{"id":352289,"name":"Bret N. Peterson","orcid":null,"position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":null,"created_at":"2026-07-18T21:14:22.090509Z","pmid":"32843560","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}