{"doi":"10.1128/mbio.01388-22","title":"Fatty Acid Synthesis Knockdown Promotes Biofilm Wrinkling and Inhibits Sporulation in Bacillus subtilis","abstract":"For many bacteria, life typically involves growth in dense, three-dimensional communities called biofilms that contain cells with differentiated roles held together by extracellular matrix. To examine how essential gene function varies between vegetative growth and the developmental states of biofilm formation and sporulation, we created and screened a comprehensive library of strains using CRISPRi to knockdown expression of each essential gene in the biofilm-capable Bacillus subtilis strain 3610. High-throughput assays and computational algorithms identified a subset of essential genes involved in biofilm wrinkling and sporulation and indicated that fatty acid synthesis plays important and multifaceted roles in bacterial development.","journal":"mBio","year":2022,"id":276967,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9486,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":584833,"name":"Haiwen Gui","orcid":"0000-0003-0564-940X","position":1,"is_corresponding":false},{"id":947944,"name":"Rachel Porter","orcid":"0000-0002-2594-9349","position":2,"is_corresponding":false},{"id":318950,"name":"Esha Atolia","orcid":null,"position":3,"is_corresponding":false},{"id":259592,"name":"Jason M. Peters","orcid":"0000-0003-2722-8382","position":4,"is_corresponding":false},{"id":259595,"name":"Carol A. Gross","orcid":"0000-0002-5595-9732","position":5,"is_corresponding":false},{"id":310236,"name":"Daniel B. Kearns","orcid":"0000-0002-3460-8378","position":6,"is_corresponding":false},{"id":74113,"name":"Kerwyn Casey Huang","orcid":"0000-0002-8043-8138","position":7,"is_corresponding":false},{"id":310231,"name":"Heidi A. Arjes","orcid":"0000-0002-4708-4286","position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":null,"created_at":"2026-07-19T00:28:34.768552Z","pmid":"36069446","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}