{"doi":"10.1128/mbio.00633-22","title":"TrmB Family Transcription Factor as a Thiol-Based Regulator of Oxidative Stress Response","abstract":"TrmB-like proteins, while not yet associated with redox stress, are found in bacteria and widespread in archaea. Here, we expand annotation of a large group of TrmB-like single winged-helix DNA binding domain proteins from diverse archaea to function as thiol-based transcriptional regulators of oxidative stress response. Using Haloferax volcanii as a model, we reveal that the TrmB-like OxsR functions during hypochlorite stress as a transcriptional activator and repressor of an extensive gene coexpression network associated with thiol relay and other related activities. A conserved cysteine residue of OxsR serves as the thiol-based sensor for this function and likely forms an intersubunit disulfide bond during hypochlorite stress that stabilizes a homodimeric configuration with enhanced DNA binding properties. A CG-rich DNA motif in the promoter region of a subset of sites identified to be OxsR-bound is required for regulation; however, not all sites have this motif, suggesting added complexity to the regulatory network.","journal":"mBio","year":2022,"id":254554,"datarank":0.4636563680037475,"base_score":3.091042453358316,"endowment":3.091042453358316,"self_citation_contribution":0.4636563680037475,"citation_network_contribution":0.0,"self_endowment_contribution":0.4636563680037475,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9488,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":289104,"name":"Sungmin Hwang","orcid":"0000-0001-8347-8656","position":1,"is_corresponding":false},{"id":767321,"name":"Lakshmi Kasirajan","orcid":"0000-0001-6620-9841","position":2,"is_corresponding":false},{"id":901672,"name":"Rebecca Oyetoro","orcid":"0000-0002-0139-5492","position":3,"is_corresponding":false},{"id":902295,"name":"Angelina Nasthas","orcid":null,"position":4,"is_corresponding":false},{"id":902296,"name":"Emily Winters","orcid":null,"position":5,"is_corresponding":false},{"id":767322,"name":"Ricardo L. Couto-Rodríguez","orcid":"0000-0002-4222-9538","position":6,"is_corresponding":false},{"id":488953,"name":"Amy Schmid","orcid":"0000-0001-5821-8000","position":7,"is_corresponding":false},{"id":345489,"name":"Julie A. Maupin‐Furlow","orcid":"0000-0001-6105-0923","position":8,"is_corresponding":false},{"id":767749,"name":"Paula Mondragon","orcid":null,"position":0,"is_corresponding":true}],"reference_count":121,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:25:08.518416Z","pmid":"35856564","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}