{"doi":"10.1128/mbio.00393-22","title":"Harnessing Rare Actinomycete Interactions and Intrinsic Antimicrobial Resistance Enables Discovery of an Unusual Metabolic Inhibitor","abstract":"Many antibiotics were originally discovered from microbes. However, in recent decades, resistance to current treatments has risen, while novel antibiotic discovery has become increasingly challenging. Thus, there is a need to develop new strategies to find novel antimicrobials. Here, we incorporated three levels of innovation into a single, simple discovery pipeline: focusing on understudied bacteria with a high potential for producing antibiotics, growing these bacteria in binary microbial interactions, and screening for activity against a multidrug-resistant bacterium. This led us to discover a family of antimicrobials that we call the dynaplanins, which are synthesized by genes that were not detected by typical prediction algorithms. We found that dynaplanins likely block the function of one of three related enzymes called 2-oxo acid dehydrogenases, which are vital to cellular metabolism. Overall, our strategy based on bacterial competition led to discovery of a novel antibiotic that inhibits the ability to metabolize nutrients.","journal":"mBio","year":2022,"id":274067,"datarank":0.5997607435990688,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.24007645267931313,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.24007645267931313,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":10,"citers_with_citation_signal":7,"citers_with_endowment":7,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.948,"is_data_producer":true,"deposit_databanks":{"figshare":["10.6084/m9.figshare.19252106.v1"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":402288,"name":"Yongle Du","orcid":"0000-0002-5727-3355","position":1,"is_corresponding":false},{"id":942636,"name":"Dominic J. Antonich","orcid":null,"position":2,"is_corresponding":false},{"id":741528,"name":"Bailey Bonet","orcid":null,"position":3,"is_corresponding":false},{"id":402266,"name":"Wenjun Zhang","orcid":"0000-0001-9348-972X","position":4,"is_corresponding":false},{"id":611934,"name":"Matthew F. Traxler","orcid":"0000-0001-8430-595X","position":5,"is_corresponding":false},{"id":942085,"name":"Dylan J. McClung","orcid":"0000-0001-7053-2676","position":0,"is_corresponding":true}],"reference_count":99,"raw_metadata":null,"created_at":"2026-07-19T00:28:07.776043Z","pmid":"35608300","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}